NOUVEAU PROCEDE DE PREPARATION DE BIOPUCES A ADN OU A PROTEINES ET LEURS APPLICATIONS

The invention relates to a method for production of an activated biochip for covalent fixing of oligonucleotide probes to a solid support by means of a spacer compound of the NHS-PEG-VS type and biochips produced by the above method. The invention further relates to methods for detection of nucleic...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	POTIER MARIE CLAUDE, ROSSIER JEAN, GIBELIN NATHALIE, TALINI LUC, NASSOY PIERRE
Format:	Patent
Sprache:	fre
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY APPARATUS THEREFOR BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY GENERAL METHODS OF ORGANIC CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for production of an activated biochip for covalent fixing of oligonucleotide probes to a solid support by means of a spacer compound of the NHS-PEG-VS type and biochips produced by the above method. The invention further relates to methods for detection of nucleic acids in a sample or methods for screening compounds which may be specifically bound to oligonucleotide probes in which said biochips are used. The invention also relates to detection kits for quantitative or qualitative analysis of nucleic acids in a sample comprising said biochips and use of the above as affinity matrix for purification of nucleic acids, for sequencing nucleic acids, for the qualitative or quantitative analysis of the expression of genes or for the study and detection of genetic polymorphism.