New nucleic acid sequences encoding enzymes involved in macrolide biosynthesis

New isolated DNA (I), single or double stranded, is a 3439 bp sequence (S1), reproduced, containing the eryG-eryAIII region of the gene cluster for erythromycin biosynthesis. Also new are (A) DNA (Ia) representing the eryAI-eryK region of the same cluster (8160 bp, S2); (B) nucleic acid correspondin...

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Hauptverfasser:	MICHEL JEAN MARC, LEADLAY PETER, SALAH BEY KHADIDJA, RAYNAL MARIE CECILE, CORTES JESUS, SALAS JOSE A, MENDEZ CARMEN, FROMENTIN CLAUDE, GAISSER SABINE
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	New isolated DNA (I), single or double stranded, is a 3439 bp sequence (S1), reproduced, containing the eryG-eryAIII region of the gene cluster for erythromycin biosynthesis. Also new are (A) DNA (Ia) representing the eryAI-eryK region of the same cluster (8160 bp, S2); (B) nucleic acid corresponding to one or more open reading frames (ORFs) within (I) or (Ia); (C) polypeptides (II) encoded by (I) and (Ia); (D) isolated DNA (Ib) representing a region of the gene cluster for oleandomycin biosynthesis (6093 bp, S3); (E) nucleic acid corresponding to one or more ORFs within (Ib); (F) polypeptides (IIa) encoded by (Ib); (G) strains of Saccharopolyspora erythrea (S.e.) in which specific enzymes are inactivated such that particular macrolides are produced; (H) method for producing oleandomycin precursors in Streptomyces antibioticus (S.a.) by altering an ORF of (S3); (J) thymidine 5'-(trihydrogen diphosphate), P'- 3,4,6-trideoxy-3-dimethylamino-D-xylohexopyranosyl ester (dTDP-D-desosamine) and its base addition salts. L'invention a pour objet la thymidine 5'-(trihydrogène diphosphate), P'-[3, 4, 6-tridésoxy-3(diméthylamino) -D-. xylo. - hexopyranosyl] ester (dTDP-D-désosamine) et les sels d'addition avec les bases.