Alpha-glucosidase and pullulanase obtained from culture of Thermococcus hydrothermalis

alpha -Glucosidase and/or pullulanase obtained by culturing a strain of Thermococcus hydrothermalis are new. The strain is preferably Thermococcus hydrothermalis CNCM I 1319 (deposited at the Collection Nationale des Cultures de Microorganisms on 16.06.1993). The T. hydrothermalis CNCM I 1319 produc...

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Hauptverfasser:	DUCHIRON FRANCIS, COPINET LEGIN ESTELLE, GANTELET HUBERT
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	alpha -Glucosidase and/or pullulanase obtained by culturing a strain of Thermococcus hydrothermalis are new. The strain is preferably Thermococcus hydrothermalis CNCM I 1319 (deposited at the Collection Nationale des Cultures de Microorganisms on 16.06.1993). The T. hydrothermalis CNCM I 1319 produces (a) alpha -glucosidase which (i) has a maximum activity at a pH 5.0-5.6 at 96-116 deg C on a substrate of starch and (ii) has an maximum activity at 110 deg C at a pH5.5 on a starch substrate and (b) pullulanase which (i) has a maximum activity at a pH 5.0-6.2 at 85-115 deg C on a starch or pullulan substrate and (ii) has a maximum activity at 110 deg C at a pH 5.5 on a substrate of pullulan or starch. La présente invention est relative à une nouvelle pullulanase obtenue à partir d'une souche du genre Thermococcus ainsi qu'à son utilisation pour transformer un amidon en un sirop de sucre.