Orange-red dye extracted from red alga - useful as colourant and immuno-fluorescence label

New orange-red dye (I) comprises a type-1 R-phycoerythrin extracted from the red alga Dilsea carnosa. Pref. (I) has UV/VIS absorption maxima at 498-500, 538-540 and 565-567nm, and fluorescence emission and excitation maxima at 575-577 and 498-502nm respectively. (I) is obtd. by macerating thalli of...

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Bibliographische Detailangaben
Hauptverfasser:	MILLE DOMINIQUE, DE ROECK HERVE, DE ROECK-HOLTZHAUER YANNICK, MILLE-CLAIRE CORINE
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	ADHESIVES CHEMISTRY DYES FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LAKES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MORDANTS NATURAL RESINS ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES PAINTS PHYSICS POLISHES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS TESTING THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
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Beschreibung
Zusammenfassung:	New orange-red dye (I) comprises a type-1 R-phycoerythrin extracted from the red alga Dilsea carnosa. Pref. (I) has UV/VIS absorption maxima at 498-500, 538-540 and 565-567nm, and fluorescence emission and excitation maxima at 575-577 and 498-502nm respectively. (I) is obtd. by macerating thalli of D.carnose, filtering, centrifuging for 20-40mins. adding (NH4)2SO4 to the supernatant, centrifuging for 20-50mins., resuspending the pellet in H2O and dialysing for 24-48hrs.. Pref. the thalli are macerated in twice their wt. of water for 4-8 weeks. The (NH4)2SO4 is added to 30-60% satn.. The dialysed soln. is freeze dried. USE/ADVANTAGE - (I) is useful as colourant in cosmetic, dietetic, food and pharmaceutical prods., and as an immunofluorescence label. (I) is water-soluble, is stable at pH 4.5-10, is stable up to 60 deg. C and can be stored in daylight for long periods without losing its colour. L'invention concerne un colorant de couleur rouge orangé constitué d'une R-phycoérythrine de type 1. Ce colorant est remarquable par le fait qu'il est extrait de l'algue rouge Dilsea carnosa. L'invention concerne également un procédé pour son obtention et ses utilisations en cosmétique, dans l'industrie alimentaire et autres, notamment à titre d'agent traceur en immunofluorescence.