SPECIFIK BINDNINGSANALYSMETOD OCH REAGENSMEDEL FOER ANVAENDNING DAERVID

A test process operating using a specific bond for the investigation of a liquid medium on a ligand is described. The test process comprises the following steps: (a) the liquid medium is brought into contact with a reagent which contains a conjugate of a labelling substance which has a predetermined characteristic and a specific binding substance, the reagent and the ligand forming a binding reaction system which (i) forms a bound phase of the labelling conjugate, in which the specific binding fraction is bound thereto by a specific binding component, and (ii) forms a free phase of the labelling conjugate, in which the specific binding fraction is not bound thereto by a specific binding component, (b) determination of the said characteristics either in the bound phase or in the free phase as a measure of the said ligands in the said liquid medium. The said characteristic of the labelling substance proves to be active in the following reaction systems: (1) as a substrate in an enzyme-catalysed reaction, (2) as a reactant in a cyclic reaction system, (3) as a reactant in a chemiluminescent reaction system, or (4) as a coenzyme in an enzyme-catalysed reaction.