Cepa modificada por ingeniería genética y aplicación de la misma en la producción de danshensu

The present disclosure discloses an engineering strain and application thereof in joint production of Danshensu and alanine, and belongs to the technical field of bioengineering. The present disclosure constructs a three-enzyme co-expression genetic engineering strain, and realizes joint production...

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Bibliographische Detailangaben
Hauptverfasser: LIU, Jinbin, BAI, Yajun, DING, Yanrui, XIONG, Tianzhen, CAI, Yujie, ZHENG, Xiaohui
Format: Patent
Sprache:spa
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Beschreibung
Zusammenfassung:The present disclosure discloses an engineering strain and application thereof in joint production of Danshensu and alanine, and belongs to the technical field of bioengineering. The present disclosure constructs a three-enzyme co-expression genetic engineering strain, and realizes joint production of Danshensu and alanine. Further, the transport of a substrate is promoted and decomposition of products is reduced by knocking out or enhancing expression of related genes on E. coli genome. The genetic engineering strain provided by the present disclosure can produce optically pure D-danshensu and L-danshensu, and jointly produce pyruvic acid. The production process is simple, raw materials are easily available, impurities are fewer, and a good industrial application prospect is achieved.