Method of extraction of biliproteins in cultures of microorganisms using glycerol solutions (Machine-translation by Google Translate, not legally binding)

Method of extracting biliproteins in cultures of microorganisms using glycerol solutions. The purpose is to obtain extracts of phycobiliproteins from microorganism cultures (such as microalgae or cyanobacteria) using an ecological solvent that is harmless to human health with higher extraction yields, fewer stages and lower energy requirements than those currently used. It works even with wet biomass. The biomass is immersed in a glycerol solution at room temperature or controlled temperature for at least 4 hours, centrifuged and the extract rich in phycobiliproteins is obtained directly or by the addition, prior to centrifugation, of a small volume of buffer solution suitable (such as the solution buffer of acetic acid sodium acetate ph 5.5 250 mm). (Machine-translation by Google Translate, not legally binding) Método de extracción de biliproteínas en cultivos de microorganismos utilizando disoluciones de glicerol. El objeto consiste en obtener extractos de ficobiliproteínas a partir de cultivos de microorganismos (como microalgas o cianobacterias) utilizando un solvente ecológico e inocuo para la salud humana con mayores rendimientos de extracción, menor número de etapas y menores requerimientos energéticos que los actualmente utilizados ya que funciona incluso con biomasa húmeda. Se sumerge la biomasa en una disolución de glicerol a temperatura ambiente o temperatura controlada durante al menos 4 horas, se centrifuga y se obtiene el extracto rico en ficobiliproteínas directamente o bien mediante la adición, previo a la centrifugación, de un pequeño volumen de disolución buffer adecuada (como el buffer de disolución de ácido acético acetato de sodio pH 5.5 250 mM).