Cumarinas sulfonadas, su síntesis, sustratos fluorogénicos que resultan de un injerto de estas cumarinas sobre azúcares, procedimiento de obtención de estos sustratos, así como sus aplicaciones

Sustrato fluorogénico que tiene por fórmula general (II)**Fórmula** en la que: - R1 representa H, u OH, o un radical alquilo de C1 a C6 sustituido o no, lineal o ramificado, o -COR4, o -COOR4, o -CONHR4, - R2 representa H, o un halógeno, en particular el flúor, o un radical alquilo de C1 a C6 sustit...

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Hauptverfasser:	DREVELLE, Antoine, LADAME, Sylvain, NAJAH, Majdi, MAYOT, Estelle
Format:	Patent
Sprache:	spa
Schlagworte:	ADHESIVES BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF DYES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MISCELLANEOUS APPLICATIONS OF MATERIALS MISCELLANEOUS COMPOSITIONS MUTATION OR GENETIC ENGINEERING NATURAL RESINS NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PAINTS PHYSICS POLISHES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Sustrato fluorogénico que tiene por fórmula general (II)Fórmula en la que: - R1 representa H, u OH, o un radical alquilo de C1 a C6 sustituido o no, lineal o ramificado, o -COR4, o -COOR4, o -CONHR4, - R2 representa H, o un halógeno, en particular el flúor, o un radical alquilo de C1 a C6 sustituido o no, lineal o ramificado, o -COR4, o -COOR4, o -CONHR4, - R1 y R2 pueden formar juntos un anillo, tal como un arilo o un furano, sustituido o no, - R3 representa H, o un halógeno, en particular el flúor, o un radical alquilo de C1 a C6 sustituido o no, lineal o ramificado, o -COR4, o -COOR4, o -CONHR4, - siendo R4 H, o un radical alquilo de C1 a C6 sustituido o no, lineal o ramificado o un arilo, sustituido o no, - M representa Na o K, - Z representa un azúcar seleccionado de entre los azúcares siguientes: celobiosa, xilobiosa, maltosa, sacarosa, glucosa, xilosa, galactosa, arabinosa, xilano, glucano, xilotriosa, maltotriosa, celotriosa, xilotetraosa, o una mezcla de algunos de ellos, - y en la que no pudiendo Z ser la galactosa cuando R1 es H, R2 y R3 son F y M es Na. Fluorogenic substrates, methods of making the fluorogenic substrates, and methods of detecting glycosidase activities (EC3.2.1) are provided. The fluorogenic substrates are composed of a sugar covalently bonded to the 7-hydroxy position of a substituted coumarin sulfonated fluorophore is provided. The fluorogenic substrates include sodium -D-cellobioside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate; sodium -D-glucoside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate; sodium -D-xyloside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate; sodium -D-xylobioside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate; and sodium -D-xylopolyoside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate. The fluorogenic substrates may be made by covalently bonding the sugar moiety to the 7-hydroxy position of the substituted coumarin sulfonated fluorophore by reaction in DMF. Glycosidase activities can be detected in enzymatic extracts, microorganisms, and cells by contacting the extracts, microorganisms, or cells with one of the fluorogenic substrates.