System for measuring sulphite in food samples using biosensor; method for the determination of sulfit in food samples using the quoted biosensor; and use of the quotation biosensor to measure the sulfit values in food samples (Machine-translation by Google Translate, not legally binding)

System to measure sulfite in food samples by means of biosensor; method for the determination of sulfite in food samples using said biosensor; and use of said biosensor to measure sulfite values in food samples. The system uses an amperometric biosensor comprising a bi-enzymatic system; a working el...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Jaureguibeitia Cayrols, Arrate, Arantzamendi Egiguren, Alai, Gonzalez Urquidi, Irune, Salleres Alonso, Sandra, Maza del Río, Sonia, González Rioja, Roberto
Format: Patent
Sprache:eng ; spa
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:System to measure sulfite in food samples by means of biosensor; method for the determination of sulfite in food samples using said biosensor; and use of said biosensor to measure sulfite values in food samples. The system uses an amperometric biosensor comprising a bi-enzymatic system; a working electrode; a counter-electrode; and a reference electrode. The bi-enzymatic system is composed of human sulfite oxidase and peroxidase enzymes; and the immobilization of the enzymes in the working electrode is carried out by simple physical retention. The mixture of the enzymes is mixed with a chemical mediator. The method comprises the phases of application of a sample to the amperometric biosensor; change of electric current upon contact with the analyte to be detected; and measuring said electric current amperometrically in solutions with continuous agitation, using a constant potential. (Machine-translation by Google Translate, not legally binding) Sistema para medir sulfito en muestras alimentarias mediante biosensor; método para la determinación de sulfito en muestras alimentarias utilizando el citado biosensor; y uso del citado biosensor para medir los valores de sulfito en muestras alimentarias. El sistema utiliza un biosensor amperométrico que comprende un sistema bi-enzimático; un electrodo de trabajo; un contra-electrodo; y un electrodo de referencia. El sistema bi-enzimático está compuesto por las enzimas sulfito oxidasa humana y peroxidasa; y la inmovilización de las enzimas en el electrodo de trabajo se lleva a cabo mediante simple retención física. La mezcla de las enzimas se mezcla a su vez con un mediador químico. El método comprende las fases de aplicación de una muestra al biosensor amperométrico; cambio de corriente eléctrica al entrar en contacto con el analito a detectar; y medir amperométricamente dicha corriente eléctrica en soluciones con agitación continua, utilizando un potencial constante.