Mutante de aminasa 5'-xmp específico de amoníaco

Mutante de aminasa 5'-xmp específico de amoníaco

Un mutante de aminasa 5'-XMP específico de amoníaco, preparado sustituyendo cisteína en la posición 86 de aminasa 5'-XMP con alanina, serina o glicina, en el que la aminasa 5'-XMP tiene una secuencia de aminoácidos seleccionada del grupo que consiste en SEQ ID NO.: 2, 4, 6, 8, 10, 12...

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Hauptverfasser: SIM, JAE ICK, LEE, JIN NAM, CHOI, KYUNG OH, PAN, JAE-GU, JUNG, HEUNGAE, KIM, HYOUNG SUK, KIM, HYUN SOO, LEE, HAN-SEUNG, OH, YOON-SUK, PARK, YOUNG HOON, HONG, KUK-KI, OH, KI-HOON, KIM, EUI-JOONG, KANG, TAE SUN, KIM, JEONG HWAN, BAEK, MIN-JI, HAN, JONG-KWON
Format: Patent
Sprache:spa
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Zusammenfassung:Un mutante de aminasa 5'-XMP específico de amoníaco, preparado sustituyendo cisteína en la posición 86 de aminasa 5'-XMP con alanina, serina o glicina, en el que la aminasa 5'-XMP tiene una secuencia de aminoácidos seleccionada del grupo que consiste en SEQ ID NO.: 2, 4, 6, 8, 10, 12 y 14. Disclosed herein are ammonia-specific 5'-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5'-XMP aminase. The resulting 5'-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5'-XMP into 5'-GMP. Thus, the ammonia-specific 5'-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5'-XMP into 5'-GMP.