Biomolecule purification procedure using expanded bed adsorption chromatography (Machine-translation by Google Translate, not legally binding)

A method of purification of biomolecules using expanded bed adsorption chromatography, which comprises introducing discontinuously with an elastic membrane pulsator a biological extract in said expanded bed comprising an adsorbent resin, washing the non-adsorbed molecules in said adsorbent resin and eluting biomolecules adsorbed on said adsorbent resin. The process of the invention can be applied to the purification of b-phycoerythrin from protein extract of the microalga phorphyridium cruentum. (Machine-translation by Google Translate, not legally binding) Un procedimiento de purificación de biomoléculas que utiliza cromatografía de adsorción en lecho expandido, que comprende introducir de forma discontinua con un pulsador de membrana elástica un extracto biológico en dicho lecho expandido que comprende una resina adsorbente, lavar las moléculas no adsorbidas en dicha resina adsorbente y eluir las biomoléculas adsorbidas en dicha resina adsorbente. El procedimiento de la invención puede aplicarse a la purificación de B-ficoeritrina a partir de extracto de proteínas de la microalga Phorphyridium cruentum.