Procedure to eliminate dopamine from different sources (Machine-translation by Google Translate, not legally binding)

Procedure to eliminate dopamine from different sources. The procedure is based on the reactions catalyzed by the genes present in the tyn and hpa gene clusters of pseudomonas putida u, starting from transforming tyramine or dopamine into 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyd...

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Bibliographische Detailangaben
Hauptverfasser:	LUENGO RODRIGUEZ, JOSE MARIA, RODRIGUEZ OLIVERA, ELIAS, ARCOS RODRIGUEZ, MARIO, NAHARRO CARRASCO, GERMAN
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	CHEMICAL RIPENING OF FRUIT OR VEGETABLES DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES MAKING THEREOF MILK OR CHEESE SUBSTITUTES PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT,VEGETABLES, EDIBLE SEEDS THE PRESERVED, RIPENED, OR CANNED PRODUCTS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
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Beschreibung
Zusammenfassung:	Procedure to eliminate dopamine from different sources. The procedure is based on the reactions catalyzed by the genes present in the tyn and hpa gene clusters of pseudomonas putida u, starting from transforming tyramine or dopamine into 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde, a reaction that can be continued to the 4-hydroxy acids. Hydroxyphenylacetic and 3,4-dihydroxyphenylacetic, respectively, by the enzymes of the cluster tyn and that, if complemented with the enzymes of the hpa cluster, allows the degradation of tyramine and dopamine in pyruvic acid and succinic acid. The invention also relates to the enzymes that catalyze the process, nucleic acid molecules that encode them, vectors that allow their expression, and especially to recombinant microorganisms transformed with said vectors. In addition, the invention relates to the use of microorganisms possessing the appropriate genes to express the proteins involved in the process to reduce the amount of food dopamine. (Machine-translation by Google Translate, not legally binding) Procedimiento para eliminar dopamina de diferentes fuentes. El procedimiento se basa en las reacciones catalizadas por los genes presentes en las agrupaciones génicas tyn y hpa de Pseudomonas putida U, partiendo de transformar tiramina o dopamina en 4-hidroxifenilacetaldehído y 3,4-dihidroxifenilacetaldehído, reacción que puede continuarse a los ácidos 4-hidroxifenilacético y 3,4-dihidroxifenilacético respectivamente, mediante las enzimas del cluster tyn y que, si se complementa con las enzimas del cluster hpa, permite la degradación de tiramina y dopamina en ácido pirúvico y ácido succínico. La invención se refiere además a las enzimas que catalizan el proceso, moléculas de ácido nucleíco que las codifican, vectores que permitan su expresión y, especialmente a microorganismos recombinantes transformados con dichos vectores. Además, la invención se refiere al uso de microorganismos que posean los genes adecuados para expresar las proteínas implicadas en el proceso para reducir la cantidad de dopamina de alimentos.