New tiramine oxidase, nucleic acid that codifies and uses the same (Machine-translation by Google Translate, not legally binding)

New tyramine oxidase, nucleic acid encoding and use thereof. The new tyramine oxidase is part of the enzymes encoded by the genes present in the tyn and hpa gene clusters of pseudomonas putida u, implicated in a degradation pathway of tyramine and/or dopamine hitherto unknown. Said tyramine oxidase...

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Bibliographische Detailangaben
Hauptverfasser:	LUENGO RODRIGUEZ, JOSE MARIA, RODRIGUEZ OLIVERA, ELIAS, ARCOS RODRIGUEZ, MARIO, NAHARRO CARRASCO, GERMAN
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL RIPENING OF FRUIT OR VEGETABLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE ENZYMOLOGY FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES MAKING THEREOF METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MILK OR CHEESE SUBSTITUTES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT,VEGETABLES, EDIBLE SEEDS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THE PRESERVED, RIPENED, OR CANNED PRODUCTS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	New tyramine oxidase, nucleic acid encoding and use thereof. The new tyramine oxidase is part of the enzymes encoded by the genes present in the tyn and hpa gene clusters of pseudomonas putida u, implicated in a degradation pathway of tyramine and/or dopamine hitherto unknown. Said tyramine oxidase transforms tyramine or dopamine into 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde, thereby allowing to reduce the amount of tyramine and/or dopamine in foods. The invention also relates to the nucleic acid molecules encoding the enzyme, vectors that allow its expression, and especially to recombinant microorganisms transformed with said vectors. In addition, the invention relates to the use of the enzyme, the nucleic acid molecules that encode it, the vectors that allow its expression, and, especially, the recombinant microorganisms that express said enzyme to reduce the amount of tyramine and/or dopamine in foods. (Machine-translation by Google Translate, not legally binding) Nueva tiramina oxidasa, ácido nucleico que la codifica y uso de los mismos. La nueva tiramina oxidasa forma parte de las enzimas codificadas por los genes presentes en las agrupaciones génicas tyn y hpa de Pseudomonas putida U, implicados en una vía de degradación de tiramina y/o dopamina desconocida hasta ahora. Dicha tiramina oxidasa transforma tiramina o dopamina en 4-hidroxifenilacetaldehído y 3,4-dihidroxifenilacetaldehído, por lo que permite reducir la cantidad de tiramina y/o dopamina en alimentos. La invención se refiere también a las moléculas de ácido nucleico que codifican la enzima, vectores que permitan su expresión y, especialmente a microorganismos recombinantes transformados con dichos vectores. Además, la invención se refiere al uso de la enzima, las moléculas de ácido nucleico que la codifican, los vectores que permiten su expresión, y, especialmente, los microorganismos recombinantes que expresen dicha enzima para reducir la cantidad de tiramina y/o dopamina en alimentos.