SISTEMA DE AUTOLISIS CELULAR PARA EL PROCESADO DE LA BIOMASA BACTERIANA EN LA PRODUCCION DE POLIHIDROXIALCANOATOS EN PSEUDOMONAS PUTIDA KT2440

The present invention relates to a bacterial strain of Pseudomonas putida KT2440, which includes a lytic heterologous genetic system in which said system in turn includes the nucleotide sequence that encodes the lytic enzyme with endolysin cell wall Ejl, the nucleotide sequence that encodes holin Ej...

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Bibliographische Detailangaben
Hauptverfasser:	GARCIA GONZALEZ, PEDRO, PRIETO JIMENEZ, MARIA AUXILIADORA, MARTINEZ LOPEZ, VIRGINIA, DUQUE MARTIN DE OLIVA, ESTRELLA, RAMOS MARTIN, JUAN LUIS, DIAZ FERNANDEZ, EDUARDO, GARCIA LOPEZ, JOSE LUIS
Format:	Patent
Sprache:	spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a bacterial strain of Pseudomonas putida KT2440, which includes a lytic heterologous genetic system in which said system in turn includes the nucleotide sequence that encodes the lytic enzyme with endolysin cell wall Ejl, the nucleotide sequence that encodes holin Ejh, and a nucleotide sequence that encodes a gene regulation system, which in turn includes a nucleotide sequence promoting the gene expression, and a nucleotide sequence that encodes and expresses a protein regulating said gene expression. The strain is preferably the microorganism with deposit number CECT 7659. The present invention further relates to said strain in which the Pseudomonas putida KT2440 microorganism is mutant in one or more of the tol-pal genes. Said strain is preferably the microorganism with deposit number CECT 7658. The present invention further relates to the use of any of the described strains for extracting polyhydroxyalkanoate (PHA) synthesised by said bacterium as well as to a method for extracting said compound.