METODO Y KIT PARA LA DETECCION DEL VIRUS DE LA HEPATITIS E (VHE)

In the present invention there is described a combination of primers and a method for molecular detection of the different circulating strains of the hepatitis E virus (HEV) in humans and animal reservoirs distributed geographically throughout the world. Amplification of the HEV genome is realised b...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: FOGEDA MORENO, MARTA, POZO SANCHEZ, FRANCISCO, AVELLON CALVO, ANA, ECHEVARRIA MAYO, JOSE MANUEL
Format: Patent
Sprache:spa
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Zusammenfassung:In the present invention there is described a combination of primers and a method for molecular detection of the different circulating strains of the hepatitis E virus (HEV) in humans and animal reservoirs distributed geographically throughout the world. Amplification of the HEV genome is realised by nested RT-PCRs wherein the primers employed amplify two fragments of the ORF1 (Open Reading Frame) and ORF2 regions of the genome of this virus. Furthermore the present invention includes a kit including the combination of the aforementioned primers and other components.