METODO Y KIT PARA LA DETECCION E IDENTIFICACION DE ESTIRPES DE ESCHERICHIA COLI DIARREAGENICOS

Method and kit for the detection and identification of diarrheal escherichia coli strains. The present invention consists of a method and a kit for the detection and identification and genotyping of pathogenic strains of escherichia coli causing diarrhea (diarrhea e. Coli), and in particular verotoxin-producing e. Coli (verotoxigenic e. Coli). The body of the invention consists of a group of molecular probes for the identification of strains of verotoxigenic e. Coli and other diarreagenic e. Coli strains. These probes correspond to specific sequences of genetic variants of one or more genes of the lee pathogenicity island. The invention provides specific probes for at least 25 variants of the intimin gene (eae), 4 of the tir gene, 4 of the espa gene, 3 of espb and 3 of espd. These probes are placed in the form of an ordered array (microarray) on solid surfaces (biochip), or they are supplied to perform specific amplifications by pcr. The invention provides control dna for each of the strains. (Machine-translation by Google Translate, not legally binding)