CELLULAR CRYOPRESERVATION BY MEANS OF VITRIFICATION WITH LOW CONCENTRATIONS OF CRYOPROTECTOR

The subject of the present invention concerns a novel cellular cryopreservation method which achieves vitrification of biological samples with presence of low concentrations of cryoprotector. The method is based on use of forced convection for the purpose of increasing heat transfer and attaining ve...

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Bibliographische Detailangaben
Hauptverfasser:	SAENZ CUESTA JAIME LUIS, RISCO DELGADO RAMON, OLMO FERNANDEZ ALBERTO
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The subject of the present invention concerns a novel cellular cryopreservation method which achieves vitrification of biological samples with presence of low concentrations of cryoprotector. The method is based on use of forced convection for the purpose of increasing heat transfer and attaining very high rates of cooling and reheating which prevent nucleation and growth of ice crystals. Criopreservación celular por vitrificación con bajas concentraciones de crioprotector. El objeto de la presente invención se refiere a un nuevo procedimiento de criopreservación celular, que consigue la vitrificación de las muestras biológicas con presencia de bajas concentraciones de crioprotecto. El procedimiento se basa en el uso de la convección forzada con el objetivo de aumentar la transferencia de calor y alcanzar muy altos ritmos de enfriamiento y recalentamiento que eviten la nucleación y el crecimiento de cristales de hielo.