BRUSHITA COMO SISTEMA DE INMOVILIZACION DE ENZIMAS Y SU USO EN BIOSENSORES AMPEROMETRICOS BIOCOMPATIBLES

Brushita as an immobilization system for enzymes and its use in biocompatible amperometric biosensors. The present invention relates to a new system of enzymatic immobilization using a biocompatible inorganic material (brushite, a cement set of calcium phosphate) as a matrix for the adsorption of en...

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Bibliographische Detailangaben
Hauptverfasser:	TAMIMI MARINO FALEH, LOPEZ CABARCOS ENRIQUE, LOPEZ RUIZ BEATRIZ, SANCHEZ-PANIAGUA LOPEZ MARTA
Format:	Patent
Sprache:	spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF COMPOUNDS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INORGANIC CHEMISTRY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NON-METALLIC ELEMENTS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Brushita as an immobilization system for enzymes and its use in biocompatible amperometric biosensors. The present invention relates to a new system of enzymatic immobilization using a biocompatible inorganic material (brushite, a cement set of calcium phosphate) as a matrix for the adsorption of enzymes and an amperometric biosensor prepared with said immobilization system. It comprises the manufacturing process of the biosensor and its application in aqueous and predominantly organic media. In the manufacturing process of the biosensor, first a brushite-enzyme mixture is deposited on the electrode surface and it is allowed to dry in the air, at room temperature. Subsequently, the intermolecular cross-linking of the enzyme adsorbed to the matrix is carried out, keeping the modified electrode in glutaraldehyde vapor. The resulting biosensor is used for the determination of analytes in aqueous solvents and predominantly organic media. (Machine-translation by Google Translate, not legally binding)