METODO RAPIDO DE DETECCION Y EVALUACION DE AGENTES GENOTOXICOS

Rapid method of detection and evaluation of genotoxic agents by rt-pcr based on the induction of the lytic cycle of bacteriophages that detects and quantifies the ability of certain physical conditions and chemical compounds to induce alterations in the genetic material. The technique is based on th...

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Bibliographische Detailangaben
Hauptverfasser:	SUAREZ FERNANDEZ JUAN EVARISTO, SOBERON MALTOS NORA ELSA
Format:	Patent
Sprache:	spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Rapid method of detection and evaluation of genotoxic agents by rt-pcr based on the induction of the lytic cycle of bacteriophages that detects and quantifies the ability of certain physical conditions and chemical compounds to induce alterations in the genetic material. The technique is based on the detection of the release of exogenous lysogenic bacteria by pcr or rt-pcr, using as primers oligonucleotides that flank the attp region of the phage. The method uses gram-positive or gram-negative bacteria to increase the range of agents to be tested. This methodology is applicable to the detection of environmental genotoxicity and that of new therapeutic compounds. (Machine-translation by Google Translate, not legally binding)