Method for producing hydroxyacyl-n-phenylalkanoic acid or its salts by genetically modified strain of Pseudomonas putida, for use as antibacterials, involves developing recombinant Pseudomonas putida bacterium

The hydroxyacyl-n-phenylalkanoic acid or its salts producing method involves developing a recombinant bacterium of Pseudomonas putida that inhibits the beta-oxidation metabolic pathway so that polyhydroxyalkanoates (PHAs) are accumulated intracellularly. A gene coding for the depolymerase enzyme responsible for the mobilization of PHAs in the bacterium and release of 3-hydroxyacyl-n-phenylalkanoic acid in a culture medium is also overexpressed. The hydroxyacyl-n-phenylalkanoic acid or its salts producing method involves developing a recombinant bacterium of Pseudomonas putida that inhibits the beta-oxidation metabolic pathway so that polyhydroxyalkanoates (PHAs) are accumulated intracellularly. A gene coding for the depolymerase enzyme responsible for the mobilization of PHAs in the bacterium and release of 3-hydroxyacyl-n-phenylalkanoic acid in a culture medium is also overexpressed. Producción de ácidos 3-hidroxiacil-n-fenilalcanoicos o de sus sales mediante una cepa de Pseudomonas putida manipulada genéticamente para tal fin. La invención consiste en producir una bacteria recombinante que tenga inhibida la ruta metabólica de la {be}-oxidación de forma que acumule polihidroxialcanoatos (PHAs) intracelularmente y que, al mismo tiempo, sobreexprese el gen que codifica para el enzima despolimerasa responsable de la movilización de los PHAs en dicha bacteria y la liberación al caldo de cultivo de 3-hidroxiacil-n-fenilalcanoatos. Los compuestos liberados al medio son útiles como antibacterianos.