ENZIMAS CONDROITIN LIASA
LA PRESENTE INVENCION DESCRIBE UN METODO DE PRODUCCION DE DOS ENZIMAS ALTAMENTE PURIFICADAS, CAPACES DE DEGRADAR POLISACARIDOS DE SULFATO DE CONDROITINA. SE DESTACA UN METODO DE PURIFICACION EN MULTIPLES ETAPAS, QUE INCORPORA RUPTURA CELULAR, CROMATOGRAFIA DE INERCAMBIO CATIONICO, CROMATOGRAFIA DE A...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | spa |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | LALIBERTE, MARYSE ZIMMERMANN, JOSEPH TKALEC, ANNA LYDIA BENNETT, D.CLARK FINK, DOMINIQUE GU, KANGFU |
| description | LA PRESENTE INVENCION DESCRIBE UN METODO DE PRODUCCION DE DOS ENZIMAS ALTAMENTE PURIFICADAS, CAPACES DE DEGRADAR POLISACARIDOS DE SULFATO DE CONDROITINA. SE DESTACA UN METODO DE PURIFICACION EN MULTIPLES ETAPAS, QUE INCORPORA RUPTURA CELULAR, CROMATOGRAFIA DE INERCAMBIO CATIONICO, CROMATOGRAFIA DE AFINIDAD, CROMATOGRAFIA DE HIDROXILAPATITO, CROMATOGRAFIA DE INTERCAMBIO IONICO DE ALTA RESOLUCION, Y EXCLUSION POR TAMAÑOS. SE AISLARON ASI UNA PROTEINA DE 77000 -+ 5000 DALTON, CAPAZ DE DEGRADAR SULFATOS DE CONDROITINA A Y C, Y UNA PROTEINA DE 55000 + 2300 DALTON, CAPAZ DE DEGRADAR SULFATO DE DERMATAN. LOS GENES QUE CODIFICAN PARA DICHAS ENZIMAS, CONDROITINASA AC Y CONDROITINASA B, RESPECTIVAMENTE, SE HAN CLONADO Y SE DESCRIBEN METODOS PARA SU UTILIZACION.
The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography, hydroxylapatite chromatography, high resolution ion exchange chromatography and size exclusion is outlined. A 77,000 ± 5,000 Dalton protein capable of degrading chondroitin sulfates A and C and a 55,000 ± 2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use are described. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_ES2255062TT3</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ES2255062TT3</sourcerecordid><originalsourceid>FETCH-epo_espacenet_ES2255062TT33</originalsourceid><addsrcrecordid>eNrjZJBw9Yvy9HUMVnD293MJ8vcM8fRT8PF0DHbkYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxrsFGRqamBmZGISHGxsSoAQABLyAn</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>ENZIMAS CONDROITIN LIASA</title><source>esp@cenet</source><creator>LALIBERTE, MARYSE ; ZIMMERMANN, JOSEPH ; TKALEC, ANNA LYDIA ; BENNETT, D.CLARK ; FINK, DOMINIQUE ; GU, KANGFU</creator><creatorcontrib>LALIBERTE, MARYSE ; ZIMMERMANN, JOSEPH ; TKALEC, ANNA LYDIA ; BENNETT, D.CLARK ; FINK, DOMINIQUE ; GU, KANGFU</creatorcontrib><description>LA PRESENTE INVENCION DESCRIBE UN METODO DE PRODUCCION DE DOS ENZIMAS ALTAMENTE PURIFICADAS, CAPACES DE DEGRADAR POLISACARIDOS DE SULFATO DE CONDROITINA. SE DESTACA UN METODO DE PURIFICACION EN MULTIPLES ETAPAS, QUE INCORPORA RUPTURA CELULAR, CROMATOGRAFIA DE INERCAMBIO CATIONICO, CROMATOGRAFIA DE AFINIDAD, CROMATOGRAFIA DE HIDROXILAPATITO, CROMATOGRAFIA DE INTERCAMBIO IONICO DE ALTA RESOLUCION, Y EXCLUSION POR TAMAÑOS. SE AISLARON ASI UNA PROTEINA DE 77000 -+ 5000 DALTON, CAPAZ DE DEGRADAR SULFATOS DE CONDROITINA A Y C, Y UNA PROTEINA DE 55000 + 2300 DALTON, CAPAZ DE DEGRADAR SULFATO DE DERMATAN. LOS GENES QUE CODIFICAN PARA DICHAS ENZIMAS, CONDROITINASA AC Y CONDROITINASA B, RESPECTIVAMENTE, SE HAN CLONADO Y SE DESCRIBEN METODOS PARA SU UTILIZACION.
The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography, hydroxylapatite chromatography, high resolution ion exchange chromatography and size exclusion is outlined. A 77,000 ± 5,000 Dalton protein capable of degrading chondroitin sulfates A and C and a 55,000 ± 2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use are described.</description><edition>7</edition><language>spa</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2006</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060616&DB=EPODOC&CC=ES&NR=2255062T3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25546,76297</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060616&DB=EPODOC&CC=ES&NR=2255062T3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LALIBERTE, MARYSE</creatorcontrib><creatorcontrib>ZIMMERMANN, JOSEPH</creatorcontrib><creatorcontrib>TKALEC, ANNA LYDIA</creatorcontrib><creatorcontrib>BENNETT, D.CLARK</creatorcontrib><creatorcontrib>FINK, DOMINIQUE</creatorcontrib><creatorcontrib>GU, KANGFU</creatorcontrib><title>ENZIMAS CONDROITIN LIASA</title><description>LA PRESENTE INVENCION DESCRIBE UN METODO DE PRODUCCION DE DOS ENZIMAS ALTAMENTE PURIFICADAS, CAPACES DE DEGRADAR POLISACARIDOS DE SULFATO DE CONDROITINA. SE DESTACA UN METODO DE PURIFICACION EN MULTIPLES ETAPAS, QUE INCORPORA RUPTURA CELULAR, CROMATOGRAFIA DE INERCAMBIO CATIONICO, CROMATOGRAFIA DE AFINIDAD, CROMATOGRAFIA DE HIDROXILAPATITO, CROMATOGRAFIA DE INTERCAMBIO IONICO DE ALTA RESOLUCION, Y EXCLUSION POR TAMAÑOS. SE AISLARON ASI UNA PROTEINA DE 77000 -+ 5000 DALTON, CAPAZ DE DEGRADAR SULFATOS DE CONDROITINA A Y C, Y UNA PROTEINA DE 55000 + 2300 DALTON, CAPAZ DE DEGRADAR SULFATO DE DERMATAN. LOS GENES QUE CODIFICAN PARA DICHAS ENZIMAS, CONDROITINASA AC Y CONDROITINASA B, RESPECTIVAMENTE, SE HAN CLONADO Y SE DESCRIBEN METODOS PARA SU UTILIZACION.
The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography, hydroxylapatite chromatography, high resolution ion exchange chromatography and size exclusion is outlined. A 77,000 ± 5,000 Dalton protein capable of degrading chondroitin sulfates A and C and a 55,000 ± 2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use are described.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2006</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZJBw9Yvy9HUMVnD293MJ8vcM8fRT8PF0DHbkYWBNS8wpTuWF0twMim6uIc4euqkF-fGpxQWJyal5qSXxrsFGRqamBmZGISHGxsSoAQABLyAn</recordid><startdate>20060616</startdate><enddate>20060616</enddate><creator>LALIBERTE, MARYSE</creator><creator>ZIMMERMANN, JOSEPH</creator><creator>TKALEC, ANNA LYDIA</creator><creator>BENNETT, D.CLARK</creator><creator>FINK, DOMINIQUE</creator><creator>GU, KANGFU</creator><scope>EVB</scope></search><sort><creationdate>20060616</creationdate><title>ENZIMAS CONDROITIN LIASA</title><author>LALIBERTE, MARYSE ; ZIMMERMANN, JOSEPH ; TKALEC, ANNA LYDIA ; BENNETT, D.CLARK ; FINK, DOMINIQUE ; GU, KANGFU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_ES2255062TT33</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>spa</language><creationdate>2006</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LALIBERTE, MARYSE</creatorcontrib><creatorcontrib>ZIMMERMANN, JOSEPH</creatorcontrib><creatorcontrib>TKALEC, ANNA LYDIA</creatorcontrib><creatorcontrib>BENNETT, D.CLARK</creatorcontrib><creatorcontrib>FINK, DOMINIQUE</creatorcontrib><creatorcontrib>GU, KANGFU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LALIBERTE, MARYSE</au><au>ZIMMERMANN, JOSEPH</au><au>TKALEC, ANNA LYDIA</au><au>BENNETT, D.CLARK</au><au>FINK, DOMINIQUE</au><au>GU, KANGFU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>ENZIMAS CONDROITIN LIASA</title><date>2006-06-16</date><risdate>2006</risdate><abstract>LA PRESENTE INVENCION DESCRIBE UN METODO DE PRODUCCION DE DOS ENZIMAS ALTAMENTE PURIFICADAS, CAPACES DE DEGRADAR POLISACARIDOS DE SULFATO DE CONDROITINA. SE DESTACA UN METODO DE PURIFICACION EN MULTIPLES ETAPAS, QUE INCORPORA RUPTURA CELULAR, CROMATOGRAFIA DE INERCAMBIO CATIONICO, CROMATOGRAFIA DE AFINIDAD, CROMATOGRAFIA DE HIDROXILAPATITO, CROMATOGRAFIA DE INTERCAMBIO IONICO DE ALTA RESOLUCION, Y EXCLUSION POR TAMAÑOS. SE AISLARON ASI UNA PROTEINA DE 77000 -+ 5000 DALTON, CAPAZ DE DEGRADAR SULFATOS DE CONDROITINA A Y C, Y UNA PROTEINA DE 55000 + 2300 DALTON, CAPAZ DE DEGRADAR SULFATO DE DERMATAN. LOS GENES QUE CODIFICAN PARA DICHAS ENZIMAS, CONDROITINASA AC Y CONDROITINASA B, RESPECTIVAMENTE, SE HAN CLONADO Y SE DESCRIBEN METODOS PARA SU UTILIZACION.
The present invention describes a method for the production of two highly purified enzymes capable of degrading chondroitin sulfate polysaccharides. A multi-step purification method incorporating cell disruption, cation exchange chromatography, affinity chromatography, hydroxylapatite chromatography, high resolution ion exchange chromatography and size exclusion is outlined. A 77,000 ± 5,000 Dalton protein capable of degrading chondroitin sulfates A and C and a 55,000 ± 2,300 Dalton protein capable of degrading dermatan sulfate were isolated. The genes encoding these enzymes, chondroitinase AC and chondroitinase B, respectively, have been cloned and methods for their use are described.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | spa |
| recordid | cdi_epo_espacenet_ES2255062TT3 |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | ENZIMAS CONDROITIN LIASA |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T10%3A35%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LALIBERTE,%20MARYSE&rft.date=2006-06-16&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EES2255062TT3%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |