Identification of herbicides and of therapeutic compounds employs a genetically modified bacterian strain based on pseudomonas putida
Genetically engineered microorganism that is not capable of synthesizing a functional protein but can catalyze pathway that allows conversion of 3-hydroxy-3-methylglutaric acid (HMG) to fumaric acid and acetoacetic acid, thus when grows, it accumulates in the culture medium HMG, is new. Independent...
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| creator | ARIAS BARRAU ELSA RODRIGUEZ OLIVERA ELIAS SANDOVAL ALVAREZ ANGEL ARIAS RIVAS SAGRARIO NAHARRO CARRASCO GERMAN ARCOS RODRIGUEZ MARIO |
| description | Genetically engineered microorganism that is not capable of synthesizing a functional protein but can catalyze pathway that allows conversion of 3-hydroxy-3-methylglutaric acid (HMG) to fumaric acid and acetoacetic acid, thus when grows, it accumulates in the culture medium HMG, is new. Independent claims are: (1) a method for obtaining a strain of genetically engineered Pseudomonas putidaso that it is unable to metabolize homogentisic acid, thus accumulating in the culture medium, comprising: (a) amplification by PCR at least two DNA fragments of the genes hmgC and hmgA; (b) binding the amplified fragments and cloned into a vector that also carries a gene for antibiotic resistance resulting in a vector that carries the amplified fragments corresponding to genes hmgC and hmgA; (c) transforming the first vector and selected host microorganism transformed microorganisms resistant to the antibiotic which carried the vector; (d) transferring the vector with the amplified fragments corresponding to genes hmgC and hmgA and strain of Pseudomonas putidawith the help of a third strain of microorganism that facilitates conjugation between genetic material from the first microorganism transformed and acting donor and the genetic material of Pseudomonas putida, which acts as final host microorganism and receiver of the genetic material; and (e) selecting strains of Pseudomonas putidatransformed and unable to metabolize homogentisic acid, by growth on sucrose; and (2) testing device for identifying herbicides, for identifying algaecides, for identifying inhibitors of metabolic pathway enzymes that convert L-Phe, L-Tyr or 3-hydroxyphenylacetate homogentisic acid, comprising genetically modified microorganisms and a precursor homogenistic acid selected from: phenylalanine, tyrosine and 3-hydroxyphenylacetate or its mixtures. ACTIVITY : Herbicide; Antialgal; Metabolic; Plant Protectant. No biological data given. MECHANISM OF ACTION : None given.
Genetically engineered microorganism that is not capable of synthesizing a functional protein but can catalyze pathway that allows conversion of 3-hydroxy-3-methylglutaric acid (HMG) to fumaric acid and acetoacetic acid, thus when grows, it accumulates in the culture medium HMG, is new. Independent claims are: (1) a method for obtaining a strain of genetically engineered Pseudomonas putidaso that it is unable to metabolize homogentisic acid, thus accumulating in the culture medium, comprising: (a) amplification by PCR at least two |
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| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_ES2246718A1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ES2246718A1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_ES2246718A13</originalsourceid><addsrcrecordid>eNqNjTEKwkAQRdNYiHqHuYBFoqitSERr7cNkd6ID2Z0lsylyAO_tBDyA1Yf3H_8vi8_dU8zcscPMEkE6eNPQsmNPChj9TLIhTDRmduAkJBmjV6CQeplMghdFsg77foIg3tbIQ4su08AYQfOAHA2oYftISqOXIBEVko16XBeLDnulzS9XBVzr5-W2pSQNaUI3PzT1o6r2h2N5Ope7P5QvxPJLrA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Identification of herbicides and of therapeutic compounds employs a genetically modified bacterian strain based on pseudomonas putida</title><source>esp@cenet</source><creator>ARIAS BARRAU ELSA ; RODRIGUEZ OLIVERA ELIAS ; SANDOVAL ALVAREZ ANGEL ; ARIAS RIVAS SAGRARIO ; NAHARRO CARRASCO GERMAN ; ARCOS RODRIGUEZ MARIO</creator><creatorcontrib>ARIAS BARRAU ELSA ; RODRIGUEZ OLIVERA ELIAS ; SANDOVAL ALVAREZ ANGEL ; ARIAS RIVAS SAGRARIO ; NAHARRO CARRASCO GERMAN ; ARCOS RODRIGUEZ MARIO</creatorcontrib><description>Genetically engineered microorganism that is not capable of synthesizing a functional protein but can catalyze pathway that allows conversion of 3-hydroxy-3-methylglutaric acid (HMG) to fumaric acid and acetoacetic acid, thus when grows, it accumulates in the culture medium HMG, is new. Independent claims are: (1) a method for obtaining a strain of genetically engineered Pseudomonas putidaso that it is unable to metabolize homogentisic acid, thus accumulating in the culture medium, comprising: (a) amplification by PCR at least two DNA fragments of the genes hmgC and hmgA; (b) binding the amplified fragments and cloned into a vector that also carries a gene for antibiotic resistance resulting in a vector that carries the amplified fragments corresponding to genes hmgC and hmgA; (c) transforming the first vector and selected host microorganism transformed microorganisms resistant to the antibiotic which carried the vector; (d) transferring the vector with the amplified fragments corresponding to genes hmgC and hmgA and strain of Pseudomonas putidawith the help of a third strain of microorganism that facilitates conjugation between genetic material from the first microorganism transformed and acting donor and the genetic material of Pseudomonas putida, which acts as final host microorganism and receiver of the genetic material; and (e) selecting strains of Pseudomonas putidatransformed and unable to metabolize homogentisic acid, by growth on sucrose; and (2) testing device for identifying herbicides, for identifying algaecides, for identifying inhibitors of metabolic pathway enzymes that convert L-Phe, L-Tyr or 3-hydroxyphenylacetate homogentisic acid, comprising genetically modified microorganisms and a precursor homogenistic acid selected from: phenylalanine, tyrosine and 3-hydroxyphenylacetate or its mixtures. ACTIVITY : Herbicide; Antialgal; Metabolic; Plant Protectant. No biological data given. MECHANISM OF ACTION : None given.
Genetically engineered microorganism that is not capable of synthesizing a functional protein but can catalyze pathway that allows conversion of 3-hydroxy-3-methylglutaric acid (HMG) to fumaric acid and acetoacetic acid, thus when grows, it accumulates in the culture medium HMG, is new. Independent claims are: (1) a method for obtaining a strain of genetically engineered Pseudomonas putidaso that it is unable to metabolize homogentisic acid, thus accumulating in the culture medium, comprising: (a) amplification by PCR at least two DNA fragments of the genes hmgC and hmgA (b) binding the amplified fragments and cloned into a vector that also carries a gene for antibiotic resistance resulting in a vector that carries the amplified fragments corresponding to genes hmgC and hmgA (c) transforming the first vector and selected host microorganism transformed microorganisms resistant to the antibiotic which carried the vector (d) transferring the vector with the amplified fragments corresponding to genes hmgC and hmgA and strain of Pseudomonas putidawith the help of a third strain of microorganism that facilitates conjugation between genetic material from the first microorganism transformed and acting donor and the genetic material of Pseudomonas putida, which acts as final host microorganism and receiver of the genetic material and (e) selecting strains of Pseudomonas putidatransformed and unable to metabolize homogentisic acid, by growth on sucrose and (2) testing device for identifying herbicides, for identifying algaecides, for identifying inhibitors of metabolic pathway enzymes that convert L-Phe, L-Tyr or 3-hydroxyphenylacetate homogentisic acid, comprising genetically modified microorganisms and a precursor homogenistic acid selected from: phenylalanine, tyrosine and 3-hydroxyphenylacetate or its mixtures. ACTIVITY : Herbicide Antialgal Metabolic Plant Protectant. No biological data given. MECHANISM OF ACTION : None given.
Un método para la identificación de herbicidas y compuestos terapéuticos mediante la utilización de una cepa bacteriana manipulada genéticamente para tal fin. La invención consiste en la obtención de una cepa de Pseudomonas putida en la que se han inactivado los genes encargados de metabolizar el ácido homogenístico (Hmg) con lo que se produce una acumulación del mismo en el caldo de cultivo. El Hmg acumulado en presencia de oxígeno se oxida y polimeriza dando lugar a un pigmento rojo oscuro en el medio. En presencia de potenciales inhibidores de alguna de las etapas que intervienen en la síntesis del Hmg, la bacteria mutada no acumula el pigmento rojo. La invención es de utilidad en la identificación de herbicidas que operan a través de la ruta biosintética de Hmg, así como de posibles compuestos terapéuticos ligados a la misma, en particular las llamadas tirosinemias, de las que la alcaptonuria es la más conocida.</description><language>eng ; spa</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2006</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060216&DB=EPODOC&CC=ES&NR=2246718A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20060216&DB=EPODOC&CC=ES&NR=2246718A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ARIAS BARRAU ELSA</creatorcontrib><creatorcontrib>RODRIGUEZ OLIVERA ELIAS</creatorcontrib><creatorcontrib>SANDOVAL ALVAREZ ANGEL</creatorcontrib><creatorcontrib>ARIAS RIVAS SAGRARIO</creatorcontrib><creatorcontrib>NAHARRO CARRASCO GERMAN</creatorcontrib><creatorcontrib>ARCOS RODRIGUEZ MARIO</creatorcontrib><title>Identification of herbicides and of therapeutic compounds employs a genetically modified bacterian strain based on pseudomonas putida</title><description>Genetically engineered microorganism that is not capable of synthesizing a functional protein but can catalyze pathway that allows conversion of 3-hydroxy-3-methylglutaric acid (HMG) to fumaric acid and acetoacetic acid, thus when grows, it accumulates in the culture medium HMG, is new. Independent claims are: (1) a method for obtaining a strain of genetically engineered Pseudomonas putidaso that it is unable to metabolize homogentisic acid, thus accumulating in the culture medium, comprising: (a) amplification by PCR at least two DNA fragments of the genes hmgC and hmgA; (b) binding the amplified fragments and cloned into a vector that also carries a gene for antibiotic resistance resulting in a vector that carries the amplified fragments corresponding to genes hmgC and hmgA; (c) transforming the first vector and selected host microorganism transformed microorganisms resistant to the antibiotic which carried the vector; (d) transferring the vector with the amplified fragments corresponding to genes hmgC and hmgA and strain of Pseudomonas putidawith the help of a third strain of microorganism that facilitates conjugation between genetic material from the first microorganism transformed and acting donor and the genetic material of Pseudomonas putida, which acts as final host microorganism and receiver of the genetic material; and (e) selecting strains of Pseudomonas putidatransformed and unable to metabolize homogentisic acid, by growth on sucrose; and (2) testing device for identifying herbicides, for identifying algaecides, for identifying inhibitors of metabolic pathway enzymes that convert L-Phe, L-Tyr or 3-hydroxyphenylacetate homogentisic acid, comprising genetically modified microorganisms and a precursor homogenistic acid selected from: phenylalanine, tyrosine and 3-hydroxyphenylacetate or its mixtures. ACTIVITY : Herbicide; Antialgal; Metabolic; Plant Protectant. No biological data given. MECHANISM OF ACTION : None given.
Genetically engineered microorganism that is not capable of synthesizing a functional protein but can catalyze pathway that allows conversion of 3-hydroxy-3-methylglutaric acid (HMG) to fumaric acid and acetoacetic acid, thus when grows, it accumulates in the culture medium HMG, is new. Independent claims are: (1) a method for obtaining a strain of genetically engineered Pseudomonas putidaso that it is unable to metabolize homogentisic acid, thus accumulating in the culture medium, comprising: (a) amplification by PCR at least two DNA fragments of the genes hmgC and hmgA (b) binding the amplified fragments and cloned into a vector that also carries a gene for antibiotic resistance resulting in a vector that carries the amplified fragments corresponding to genes hmgC and hmgA (c) transforming the first vector and selected host microorganism transformed microorganisms resistant to the antibiotic which carried the vector (d) transferring the vector with the amplified fragments corresponding to genes hmgC and hmgA and strain of Pseudomonas putidawith the help of a third strain of microorganism that facilitates conjugation between genetic material from the first microorganism transformed and acting donor and the genetic material of Pseudomonas putida, which acts as final host microorganism and receiver of the genetic material and (e) selecting strains of Pseudomonas putidatransformed and unable to metabolize homogentisic acid, by growth on sucrose and (2) testing device for identifying herbicides, for identifying algaecides, for identifying inhibitors of metabolic pathway enzymes that convert L-Phe, L-Tyr or 3-hydroxyphenylacetate homogentisic acid, comprising genetically modified microorganisms and a precursor homogenistic acid selected from: phenylalanine, tyrosine and 3-hydroxyphenylacetate or its mixtures. ACTIVITY : Herbicide Antialgal Metabolic Plant Protectant. No biological data given. MECHANISM OF ACTION : None given.
Un método para la identificación de herbicidas y compuestos terapéuticos mediante la utilización de una cepa bacteriana manipulada genéticamente para tal fin. La invención consiste en la obtención de una cepa de Pseudomonas putida en la que se han inactivado los genes encargados de metabolizar el ácido homogenístico (Hmg) con lo que se produce una acumulación del mismo en el caldo de cultivo. El Hmg acumulado en presencia de oxígeno se oxida y polimeriza dando lugar a un pigmento rojo oscuro en el medio. En presencia de potenciales inhibidores de alguna de las etapas que intervienen en la síntesis del Hmg, la bacteria mutada no acumula el pigmento rojo. La invención es de utilidad en la identificación de herbicidas que operan a través de la ruta biosintética de Hmg, así como de posibles compuestos terapéuticos ligados a la misma, en particular las llamadas tirosinemias, de las que la alcaptonuria es la más conocida.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2006</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjTEKwkAQRdNYiHqHuYBFoqitSERr7cNkd6ID2Z0lsylyAO_tBDyA1Yf3H_8vi8_dU8zcscPMEkE6eNPQsmNPChj9TLIhTDRmduAkJBmjV6CQeplMghdFsg77foIg3tbIQ4su08AYQfOAHA2oYftISqOXIBEVko16XBeLDnulzS9XBVzr5-W2pSQNaUI3PzT1o6r2h2N5Ope7P5QvxPJLrA</recordid><startdate>20060216</startdate><enddate>20060216</enddate><creator>ARIAS BARRAU ELSA</creator><creator>RODRIGUEZ OLIVERA ELIAS</creator><creator>SANDOVAL ALVAREZ ANGEL</creator><creator>ARIAS RIVAS SAGRARIO</creator><creator>NAHARRO CARRASCO GERMAN</creator><creator>ARCOS RODRIGUEZ MARIO</creator><scope>EVB</scope></search><sort><creationdate>20060216</creationdate><title>Identification of herbicides and of therapeutic compounds employs a genetically modified bacterian strain based on pseudomonas putida</title><author>ARIAS BARRAU ELSA ; RODRIGUEZ OLIVERA ELIAS ; SANDOVAL ALVAREZ ANGEL ; ARIAS RIVAS SAGRARIO ; NAHARRO CARRASCO GERMAN ; ARCOS RODRIGUEZ MARIO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_ES2246718A13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; spa</language><creationdate>2006</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ARIAS BARRAU ELSA</creatorcontrib><creatorcontrib>RODRIGUEZ OLIVERA ELIAS</creatorcontrib><creatorcontrib>SANDOVAL ALVAREZ ANGEL</creatorcontrib><creatorcontrib>ARIAS RIVAS SAGRARIO</creatorcontrib><creatorcontrib>NAHARRO CARRASCO GERMAN</creatorcontrib><creatorcontrib>ARCOS RODRIGUEZ MARIO</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ARIAS BARRAU ELSA</au><au>RODRIGUEZ OLIVERA ELIAS</au><au>SANDOVAL ALVAREZ ANGEL</au><au>ARIAS RIVAS SAGRARIO</au><au>NAHARRO CARRASCO GERMAN</au><au>ARCOS RODRIGUEZ MARIO</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Identification of herbicides and of therapeutic compounds employs a genetically modified bacterian strain based on pseudomonas putida</title><date>2006-02-16</date><risdate>2006</risdate><abstract>Genetically engineered microorganism that is not capable of synthesizing a functional protein but can catalyze pathway that allows conversion of 3-hydroxy-3-methylglutaric acid (HMG) to fumaric acid and acetoacetic acid, thus when grows, it accumulates in the culture medium HMG, is new. Independent claims are: (1) a method for obtaining a strain of genetically engineered Pseudomonas putidaso that it is unable to metabolize homogentisic acid, thus accumulating in the culture medium, comprising: (a) amplification by PCR at least two DNA fragments of the genes hmgC and hmgA; (b) binding the amplified fragments and cloned into a vector that also carries a gene for antibiotic resistance resulting in a vector that carries the amplified fragments corresponding to genes hmgC and hmgA; (c) transforming the first vector and selected host microorganism transformed microorganisms resistant to the antibiotic which carried the vector; (d) transferring the vector with the amplified fragments corresponding to genes hmgC and hmgA and strain of Pseudomonas putidawith the help of a third strain of microorganism that facilitates conjugation between genetic material from the first microorganism transformed and acting donor and the genetic material of Pseudomonas putida, which acts as final host microorganism and receiver of the genetic material; and (e) selecting strains of Pseudomonas putidatransformed and unable to metabolize homogentisic acid, by growth on sucrose; and (2) testing device for identifying herbicides, for identifying algaecides, for identifying inhibitors of metabolic pathway enzymes that convert L-Phe, L-Tyr or 3-hydroxyphenylacetate homogentisic acid, comprising genetically modified microorganisms and a precursor homogenistic acid selected from: phenylalanine, tyrosine and 3-hydroxyphenylacetate or its mixtures. ACTIVITY : Herbicide; Antialgal; Metabolic; Plant Protectant. No biological data given. MECHANISM OF ACTION : None given.
Genetically engineered microorganism that is not capable of synthesizing a functional protein but can catalyze pathway that allows conversion of 3-hydroxy-3-methylglutaric acid (HMG) to fumaric acid and acetoacetic acid, thus when grows, it accumulates in the culture medium HMG, is new. Independent claims are: (1) a method for obtaining a strain of genetically engineered Pseudomonas putidaso that it is unable to metabolize homogentisic acid, thus accumulating in the culture medium, comprising: (a) amplification by PCR at least two DNA fragments of the genes hmgC and hmgA (b) binding the amplified fragments and cloned into a vector that also carries a gene for antibiotic resistance resulting in a vector that carries the amplified fragments corresponding to genes hmgC and hmgA (c) transforming the first vector and selected host microorganism transformed microorganisms resistant to the antibiotic which carried the vector (d) transferring the vector with the amplified fragments corresponding to genes hmgC and hmgA and strain of Pseudomonas putidawith the help of a third strain of microorganism that facilitates conjugation between genetic material from the first microorganism transformed and acting donor and the genetic material of Pseudomonas putida, which acts as final host microorganism and receiver of the genetic material and (e) selecting strains of Pseudomonas putidatransformed and unable to metabolize homogentisic acid, by growth on sucrose and (2) testing device for identifying herbicides, for identifying algaecides, for identifying inhibitors of metabolic pathway enzymes that convert L-Phe, L-Tyr or 3-hydroxyphenylacetate homogentisic acid, comprising genetically modified microorganisms and a precursor homogenistic acid selected from: phenylalanine, tyrosine and 3-hydroxyphenylacetate or its mixtures. ACTIVITY : Herbicide Antialgal Metabolic Plant Protectant. No biological data given. MECHANISM OF ACTION : None given.
Un método para la identificación de herbicidas y compuestos terapéuticos mediante la utilización de una cepa bacteriana manipulada genéticamente para tal fin. La invención consiste en la obtención de una cepa de Pseudomonas putida en la que se han inactivado los genes encargados de metabolizar el ácido homogenístico (Hmg) con lo que se produce una acumulación del mismo en el caldo de cultivo. El Hmg acumulado en presencia de oxígeno se oxida y polimeriza dando lugar a un pigmento rojo oscuro en el medio. En presencia de potenciales inhibidores de alguna de las etapas que intervienen en la síntesis del Hmg, la bacteria mutada no acumula el pigmento rojo. La invención es de utilidad en la identificación de herbicidas que operan a través de la ruta biosintética de Hmg, así como de posibles compuestos terapéuticos ligados a la misma, en particular las llamadas tirosinemias, de las que la alcaptonuria es la más conocida.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Identification of herbicides and of therapeutic compounds employs a genetically modified bacterian strain based on pseudomonas putida |
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