Production of a biotransformed E. coli recombinant acid consists of employing pseudomonas putida bacteria mutants and a modified E. coli recombinant strain

The production of a biotransformed E. coli recombinant acid comprises using mutants of Pseudomonas putida bacteria and an E. coli recombinant strain incorporating genes isolated from P. putida. The product is 2-hydroxyphenyl acetic acid, obtained from phenylacetic acid. The isolated genes codify the...

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Hauptverfasser:	GARCIA ALONSO BELEN, MINAMBRES RODRIGUEZ BALTASAR, FERRANDEZ BARCA ABEL, RODRIGUEZ OLIVERA ELIAS, GARCIA LOPEZ JOSE LUIS, DIAZ FERNANDEZ EDUARDO
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	The production of a biotransformed E. coli recombinant acid comprises using mutants of Pseudomonas putida bacteria and an E. coli recombinant strain incorporating genes isolated from P. putida. The product is 2-hydroxyphenyl acetic acid, obtained from phenylacetic acid. The isolated genes codify the enzymes responsible for the biotransformation. The production of a biotransformed E. coli recombinant acid comprises using mutants of Pseudomonas putida bacteria and an E. coli recombinant strain incorporating genes isolated from P. putida. The product is 2-hydroxyphenyl acetic acid, obtained from phenylacetic acid. The isolated genes codify the enzymes responsible for the biotransformation. (Machine-translation by Google Translate, not legally binding) Un procedimiento para la producción de ácido 2- hidroxifenilacético utilizando mutantes de la bacteria Pseudomonas putida U así como una cepa recombinante de E.coli en la que se han introducido genes aislados de Pseudomonas putida. La invención que se presenta, describe un procedimiento para la biotransformación del ácido fenilacético (PA) en ácido 2- hidroxifenilacético (abreviadamente indicado como 2-HPA), utilizando mutantes de la bacteria Pseudomonas putida U interrumpidos en uno de los genes (phaL) que compone la ruta catabólica de ácido fenilacético, así como el empleo de una cepa de E.coli recombinante en la que se han introducido, y se expresan, los.genes aislados de Pseudomonas putida que codifican las enzimas responsables de esa biotransformación.