Method of ultrafast detection of cow milk proteins in soy products by high-efficiency liquid chromatography

The present invention refers to a method of ultrafast detection of cow milk whey proteins by high-efficiency liquid chromatography with inverted phase perfusion, in milk type products based on soy. This method, which consists in the performance of said chromatography by the application of a linear and binary gradient in three steps, using acetonitrile-water- trifluoroacetic acid, at a flow rate determined by the time of the gradient, at constant temperature and with injection of the sample in aqueous solution, allows the rapid and simultaneous separation of soy and cow milk whey proteins in commercial products of dairy type, manufactured from soy, such as liquid and powder soy beverages (soy milk), infant formulas, yoghurts, batters, etc. The present invention refers to a method of ultrafast detection of cow milk whey proteins by high-efficiency liquid chromatography with inverted phase perfusion, in milk type products based on soy. This method, which consists in the performance of said chromatography by the application of a linear and binary gradient in three steps, using acetonitrile-water- trifluoroacetic acid, at a flow rate determined by the time of the gradient, at constant temperature and with injection of the sample in aqueous solution, allows the rapid and simultaneous separation of soy and cow milk whey proteins in commercial products of dairy type, manufactured from soy, such as liquid and powder soy beverages (soy milk), infant formulas, yoghurts, batters, etc. (Machine-translation by Google Translate, not legally binding) Procedimiento de detección ultrarrápida de proteínas de leche de vaca en productos de soja, por cromatografía líquida de alta eficacia. La presente invención se refiere a un procedimiento de detección ultrarrápida de proteínas de suero de leche de vaca por cromatografía liquida de alta eficacia de perfusión en fase inversa, en productos de tipo lácteo a partir de soja. Este procedimiento que consiste en la realización de dicha cromatografía mediante la aplicación de un gradiente lineal y binaria en tres pasos usando acetonitrilo-agua-ácido trifluoroacético, a un flujo determinado por el tiempo del gradiente, a temperatura constante y con inyección de la muestra en disolución acuosa, permite separar rápida y simultáneamente proteínas de soja y de suero de leche de vaca en productos comerciales de tipo lácteo fabricados a partir de soja tales como bebidas de soja líquidas y en polvo -leches de soja-, fórmulas infantiles, yogure