PROCEDIMIENTO DE PRODUCCION DE ENZIMA DEXTRANASA Y DECODIFICACION DE SU ADN
UN METODO PARA EL AISLAMIENTO Y LA EXPRESION DE UN GEN QUE CODIFICA LA DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM. ESTA ENZIMA DE ORIGEN FUNGICO SE PRODUCE POR EXPRESION EN ALTOS NIVELES DE LEVADURA. PARA ESTE PROPOSITO, SE AISLA Y SE SECUENCIA UNA COPIA DE ADNC DEL ARNM QUE CODIFICA LA ENZIMA DE...
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| creator | DELGADO BOADA, JULIO MARCOS HERRERA MARTINEZ, LUIS S MARGOLLEZ CLARK, EMILIO CAMPANA, HERNAN ROCA GARCIA GARCIA, BIANCA MAR A CURBELO, DANIA MATEU |
| description | UN METODO PARA EL AISLAMIENTO Y LA EXPRESION DE UN GEN QUE CODIFICA LA DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM. ESTA ENZIMA DE ORIGEN FUNGICO SE PRODUCE POR EXPRESION EN ALTOS NIVELES DE LEVADURA. PARA ESTE PROPOSITO, SE AISLA Y SE SECUENCIA UNA COPIA DE ADNC DEL ARNM QUE CODIFICA LA ENZIMA DE DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM . ESTE ADNC SE TRANSFIERE A CELULAS DE PICHIA PASTORIS. SE HAN OBTENIDO LEVADURAS RECOMBINANTES CAPACES DE SEGREGAR LA DEXTRANASA AL MEDIO DE CULTIVO. LA ENZIMA DE DEXTRANASA OBTENIDA PUEDE USARSE, POR EJEMPLO, EN LA INDUSTRIA AZUCARERA PARA HIDROLIZAR EL DEXTRANO EN JUGOS DE CAÑA PARA INCREMENTAR LA PRODUCCION DE AZUCAR.
A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production. |
| format | Patent |
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A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production.</description><edition>6</edition><language>spa</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>1998</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19980801&DB=EPODOC&CC=ES&NR=2117203T3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19980801&DB=EPODOC&CC=ES&NR=2117203T3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DELGADO BOADA, JULIO MARCOS</creatorcontrib><creatorcontrib>HERRERA MARTINEZ, LUIS S</creatorcontrib><creatorcontrib>MARGOLLEZ CLARK, EMILIO</creatorcontrib><creatorcontrib>CAMPANA, HERNAN ROCA</creatorcontrib><creatorcontrib>GARCIA GARCIA, BIANCA MAR A</creatorcontrib><creatorcontrib>CURBELO, DANIA MATEU</creatorcontrib><title>PROCEDIMIENTO DE PRODUCCION DE ENZIMA DEXTRANASA Y DECODIFICACION DE SU ADN</title><description>UN METODO PARA EL AISLAMIENTO Y LA EXPRESION DE UN GEN QUE CODIFICA LA DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM. ESTA ENZIMA DE ORIGEN FUNGICO SE PRODUCE POR EXPRESION EN ALTOS NIVELES DE LEVADURA. PARA ESTE PROPOSITO, SE AISLA Y SE SECUENCIA UNA COPIA DE ADNC DEL ARNM QUE CODIFICA LA ENZIMA DE DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM . ESTE ADNC SE TRANSFIERE A CELULAS DE PICHIA PASTORIS. SE HAN OBTENIDO LEVADURAS RECOMBINANTES CAPACES DE SEGREGAR LA DEXTRANASA AL MEDIO DE CULTIVO. LA ENZIMA DE DEXTRANASA OBTENIDA PUEDE USARSE, POR EJEMPLO, EN LA INDUSTRIA AZUCARERA PARA HIDROLIZAR EL DEXTRANO EN JUGOS DE CAÑA PARA INCREMENTAR LA PRODUCCION DE AZUCAR.
A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1998</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZPAOCPJ3dnXx9PV09QvxV3BxVQAKuIQ6O3v6-4F4rn5Rnr6OQFZESJCjn2Owo0IkkOPs7-Lp5unsCFMVHKrg6OLHw8CalphTnMoLpbkZFN1cQ5w9dFML8uNTiwsSk1PzUkviXYONDA3NjQyMQ0KMjYlRAwBtJC2e</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>DELGADO BOADA, JULIO MARCOS</creator><creator>HERRERA MARTINEZ, LUIS S</creator><creator>MARGOLLEZ CLARK, EMILIO</creator><creator>CAMPANA, HERNAN ROCA</creator><creator>GARCIA GARCIA, BIANCA MAR A</creator><creator>CURBELO, DANIA MATEU</creator><scope>EVB</scope></search><sort><creationdate>19980801</creationdate><title>PROCEDIMIENTO DE PRODUCCION DE ENZIMA DEXTRANASA Y DECODIFICACION DE SU ADN</title><author>DELGADO BOADA, JULIO MARCOS ; HERRERA MARTINEZ, LUIS S ; MARGOLLEZ CLARK, EMILIO ; CAMPANA, HERNAN ROCA ; GARCIA GARCIA, BIANCA MAR A ; CURBELO, DANIA MATEU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_ES2117203TT33</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>spa</language><creationdate>1998</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>DELGADO BOADA, JULIO MARCOS</creatorcontrib><creatorcontrib>HERRERA MARTINEZ, LUIS S</creatorcontrib><creatorcontrib>MARGOLLEZ CLARK, EMILIO</creatorcontrib><creatorcontrib>CAMPANA, HERNAN ROCA</creatorcontrib><creatorcontrib>GARCIA GARCIA, BIANCA MAR A</creatorcontrib><creatorcontrib>CURBELO, DANIA MATEU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>DELGADO BOADA, JULIO MARCOS</au><au>HERRERA MARTINEZ, LUIS S</au><au>MARGOLLEZ CLARK, EMILIO</au><au>CAMPANA, HERNAN ROCA</au><au>GARCIA GARCIA, BIANCA MAR A</au><au>CURBELO, DANIA MATEU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PROCEDIMIENTO DE PRODUCCION DE ENZIMA DEXTRANASA Y DECODIFICACION DE SU ADN</title><date>1998-08-01</date><risdate>1998</risdate><abstract>UN METODO PARA EL AISLAMIENTO Y LA EXPRESION DE UN GEN QUE CODIFICA LA DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM. ESTA ENZIMA DE ORIGEN FUNGICO SE PRODUCE POR EXPRESION EN ALTOS NIVELES DE LEVADURA. PARA ESTE PROPOSITO, SE AISLA Y SE SECUENCIA UNA COPIA DE ADNC DEL ARNM QUE CODIFICA LA ENZIMA DE DEXTRANASA DEL HONGO PENICILLIUM MINIOLUTEUM . ESTE ADNC SE TRANSFIERE A CELULAS DE PICHIA PASTORIS. SE HAN OBTENIDO LEVADURAS RECOMBINANTES CAPACES DE SEGREGAR LA DEXTRANASA AL MEDIO DE CULTIVO. LA ENZIMA DE DEXTRANASA OBTENIDA PUEDE USARSE, POR EJEMPLO, EN LA INDUSTRIA AZUCARERA PARA HIDROLIZAR EL DEXTRANO EN JUGOS DE CAÑA PARA INCREMENTAR LA PRODUCCION DE AZUCAR.
A method for the isolation and expression of a gene coding for dextranase of the fungus Penicillium minioluteum. This enzyme of fungal origin is produced by expression at high levels in yeast. For this purpose, a cDNA copy of the mRNA coding for dextranase enzyme of the Penicillium minioluteum fungus was isolated and sequenced. This cDNA was transfered into Pichia pastoris cells. Recombinant yeasts capable of secreting dextranase to the culture medium were obtained thereby. The dextranase enzyme obtained can be used, e.g. in the sugar industry to hydrolyze the dextran in cane juices to increase the sugar production.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PROCEDIMIENTO DE PRODUCCION DE ENZIMA DEXTRANASA Y DECODIFICACION DE SU ADN |
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