LIASA AMONICA DE L-FENILALANINA

PARA PROPORCIONAR LA INFORMACION TECNICA NECESARIA PARA LA PRODUCCION DE UNA LIASA AMONIACAL DE L-FENILALANINA (PAL) MEDIANTE LA UTILIZACION DE TECNICAS DE INGENIERIA GENETICA, SE HAN DILUCIDADO EL GEN ESTRUCTURAL DE LA PAL Y LA SECUENCIA AMINOACIDA DE LA PAL EN RHODOSPORIDIUM TORULOIDES Y SE HAN CREADO NUEVOS PLASMIDOS DE ADN RECOMBINANTES (EJ., PSW101, PYTRP6 Y PKY201) MEDIANTE LA INSERCION DE UNA CEPA DE ADN QUE CODIFICA AL GEN DE LA PAL ENTRE EL TERMINO 3'' DE LA REGION PROMOTORA Y EL TERMINO 5'' DE LA REGION TERMINAL. ADEMAS, SE HAN CREADO TRANSFORMADORES QUE TIENEN TAL PLASMIDO NUEVO DE ADN RECOMBINANTE Y SE HA ESTABLECIDO UN PROCESO PARA LA PRODUCCION DE LA PAL MEDIANTE EL DESARROLLO DE TAL TRANSFORMADOR NUEVO PARA ASI CONSEGUIR QUE SE PRODUZCA LA PAL Y SE ACUMULE EN EL CULTIVO. ADEMAS, SE HA ESTABLECIDO UNA NUEVA TECNICA PARA LA PRODUCCION DE L-FENILALANINA MEDIANTE LA REACCION DE UN DONANTE DE AMONIACO CON ACIDO CINAMICO ANTE LA PRESENCIA DE LA PAL PREPARADA SEGUN EL NUEVO PROCESO ANTEDICHO. In order to provide technical information necessary for the production of L-phenylalanine ammonia-lyase (PAL) by utilizing genetic engineering techniques, the structural gene for PAL and the amino acid sequence of PAL have been elucidated in Rhodosporidium toruloides, and novel recombinant DNA plasmids (e.g., pSW101, pYtrp6 and pKY201) have been created by inserting a DNA strand coding for the PAL gene between the 3'-terminus of the promoter region and the 5'-terminus of the terminator region. Moreover, transformants having such a novel recombinant DNA plasmid have been created, and a process for the production of PAL by growing such a novel transformant so as to cause PAL to be produced and accumulated in the culture has been established. Furthermore, there has been established a novel technique for the production of L-phenylalanine by reacting an ammonia donor with cinnamic acid in the presence of the PAL prepared by the aforesaid novel process.