Procedure for the purification of cyclosporin A

Procedure for the purification of cyclosporin A from mixtures which contain especially cyclosporins A, B, C and other cyclosporin components which are more polar or more apolar than cyclosporin A or contaminants similar thereto, which involves heating the mixture or an evaporation residue thereof to a temperature between 80degreeC and 115degreeC before column chromatography, melting it and subjecting the molten mass to chromatography by methods known per se. The chromatography is carried out with the successive use of the following solvent mixtures: chloroform: dichloromethane: ethanol = 48:50:2 and chloroform: ethyl acetate: ethanol = 48:50:2. PROCEDIMIENTO PARA LA PURIFICACION DE CICLOSPORINA A DE MEZCLAS QUE CONTIENEN ESPECIALMENTE CICLOSPORINAS A, B, C Y OTROS COMPONENTES DE CICLOSPORINA MAS POLARES O MAS APOLARES QUE LA CICLOSPORINA A O CONTAMINACIONES SIMILARES A ELLOS, QUE COMPRENDE CALENTAR LA MEZCLA O UN RESIDUO DE EVAPORACION DE ELLA A UNA TEMPERATURA ENTRE 80GC Y 115GC ANTES DE LA CROMATOGRAFIA DE COLUMNA, FUNDIRLA Y SOMETER LA MASA FUNDIDA A CROMATOGRAFIA MEDIANTE METODOS CONOCIDOS PER SE. LA CROMATOGRAFIA SE LLEVA A CABO CON LA UTILIZACION SUCESIVA DE LAS SIGUIENTES MEZCLAS DE DISOLVENTES: CLOROFORMO : DICLOROMETANO : ETANOL = 48:50:2 Y CLOROFORMO : ACETATO DE ETILO : ETANOL = 48:50