Viroid and virus free potato propagation material prodn

Prodn. of potato propagation material free from viruses and viroids comprises (a) isolating germinated cells of potato shoot apex tissue; (b) cultivating the cells in vitro in a nutritive agent, when they are multiplied; (c) rooting the resulting shoots in nutritive agent or inducing tuber formation on the shoots; (d) transplating the plants, rooted in vitro, into a greenhouse and raising them to become parent plants or, on the plants rooted in vitro, inducing tuber formation and if required cultivating mini tuber mother plants to give propagation material, i.e. seedlings os small tubers. The formation of seedlings and respective tubers is directed by influencing the patatin synthesis of the parent plants. Prodn. of potato propagation material free from viruses and viroids comprises (a) isolating germinated cells of potato shoot apex tissue (b) cultivating the cells in vitro in a nutritive agent, when they are multiplied (c) rooting the resulting shoots in nutritive agent or inducing tuber formation on the shoots (d) transplating the plants, rooted in vitro, into a greenhouse and raising them to become parent plants or, on the plants rooted in vitro, inducing tuber formation and if required cultivating mini tuber mother plants to give propagation material, i.e. seedlings os small tubers. The formation of seedlings and respective tubers is directed by influencing the patatin synthesis of the parent plants. (Machine-translation by Google Translate, not legally binding) LA INVENCION SE REFIERE A UN PROCEDIMIENTO DE PRODUCCION EN MASA DE MATERIAL PARA LA PROPAGACION DE LA PATATA, EXENTO DE VIRUS Y VIROIDES, POR MEDIO DEL CULTIVO DE TEJIDO DE PATATA. CONSISTE EN CULTIVAR IN VITRO, EN UN AGENTE NUTRIENTE, LAS CELULAS AISLADAS DEL TEJIDO DEL APICE DEL BROTE DE LA PATATA, DESPUES, LOS BROTES OBTENIDOS SE ENRAIZAN O SE PROVOCA EN LOS MISMOS LA FORMACION DE MINITUBERCULOS, LAS PLANTAS CON RAICES SE CULTIVAN HASTA QUE SON PLANTAS MADRE O SE PROVOCA OTRA NUEVA FORMACION DE MINITUBERCULOS QUE PRODUCEN NUEVAS PLANTAS MADRE. EL MATERIAL DE PROPAGACION PUEDE TENER FORMA DE PLANTULAS O TUBERCULOS PEQUEÑOS, PROCEDENTES DE LAS PLANTAS MADRE, O ESTAR FORMADO POR LOS PROPIOS MINITUBERCULOS.