AFFINITY CHROMATOGRAPHIC PRODUCTION OF CLINICAL HUMAN IGG PRODUCTS

An affinity chromatographic method of preparing from an IgG-containing starting material an IgG formulation that includes one or more of IgG1, IgG2, IgG3 and IgG4 is provided. A method of the invention includes contacting the IgG-containing starting material with a first chromatographic medium including a first solid support having Protein A bound thereto, forming a Protein A bound fraction of an IgG subclass with Protein A binding affinity and forming a first flow through including an IgG subclass not binding to the Protein A; and contacting the first flow through with a first affinity ligand chromatographic medium including a second solid support having a first affinity ligand with IgG binding affinity bound thereto, forming a first affinity ligand-bound IgG subclass and a second flow through including an IgG subclass not bound to the first affinity ligand-bound IgG subclass.