CHROMATOGRAPHIC PURIFICATION METHOD AND USES THEREOF

A chromatographic purification method for the isolation of a product (P) from a feed mixture (F) using two or more chromatographic columns, wherein in one step b) the first upstream column (1) is loaded with feed (F) via the inlet, followed by at least one interconnected step c) in which the columns (1,2) are interconnected in series for passing eluate containing product (P) to the second column, wherein in said interconnected step c) the first column (1) is fed with eluent with a gradient, wherein the stream exiting the first column is adjusted inline with inline adjustment eluent before entering the second column during the period of gradient elution, and wherein the inline adjustment eluent is as the eluent fed at the first column inlet but controlled to have a higher or lower modifier concentration than the eluent exiting at the first column, and wherein the modifier difference of the inline adjustment eluent is chosen such that the adherence of the product to the stationary phase of the second column (2) is higher than without that difference.