LIQUID ASPIRATION WITH VOLUME COMPENSATION

A method for aspirating a liquid (22) with a laboratory automation device (10) comprises: lowering a pipette (14) of the laboratory automation device (10) into a vessel (18) containing the liquid (22); during lowering, generating an underpressure or overpressure in the pipette (14) with a pressure g...

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Bibliographische Detailangaben
Hauptverfasser: GEIGES, Thomas, KELLER, Michael
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:A method for aspirating a liquid (22) with a laboratory automation device (10) comprises: lowering a pipette (14) of the laboratory automation device (10) into a vessel (18) containing the liquid (22); during lowering, generating an underpressure or overpressure in the pipette (14) with a pressure generating device (26) of the laboratory automation device (10) and measuring a pressure (46) in a pressure distribution system (30) interconnecting the pressure generating device (26) with the pipette (14); detecting a liquid surface (40) of the liquid (22) in the vessel (18) by evaluating a pressure change (48) of the pressure (46); calculating a compensation volume from compartment volumes of compartments (38) of the pressure distribution system (30) and/or of the pipette (14) and from pressure losses between the compartments (38); and aspirating the liquid (22) from the vessel (18) into the pipette (14) by controlling the pressure generating device (26) to aspirate a specific volume altered by the compensation volume.