METHODS AND COMPOSITIONS FOR FAST NUCLEIC ACID AMPLIFICATIONS

Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, DNA oligonucleotide containing ribonucleotide is used as primer in the presence of RNase H2. In PCR reactions, this allows a greater than 2-fold increase of amplification prod...

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Bibliographische Detailangaben
Hauptverfasser:	Dong, Christopher X, Dong, Wiliam X, Dong, Shoulian
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Described herein are methods and compositions that provide highly efficient nucleic acid amplification. In some embodiments, DNA oligonucleotide containing ribonucleotide is used as primer in the presence of RNase H2. In PCR reactions, this allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. PCR product thus generated is used for convenient cloning. In isothermal reactions, this reduces the time needed for target detection. In some embodiment, probe containing ribonucleotide is used to monitor amplification reaction without relying on the 5'-nuclease activity of DNA polymerase. The method and composition enable easy and fast detection of nucleic acid targets, enabling quick detection of genetic variations and pathogens such as SARS-CoV-2.