CELL DISSOCIATION DEVICE AND METHOD

What is described herein relates to a method and a device for dissociating cell agglomerations in the range of between >30 µm to 300 µm after harvest into single cells or cell oligomers in the range of 8-30 µm comprising the steps of* providing cell agglomerations in the range of between >30 µ...

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Bibliographische Detailangaben
Hauptverfasser: Baas, Markus, Sousa, Marcos, Tscheschke, Bernd, Poggel, Martin, Schwan, Peter
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:What is described herein relates to a method and a device for dissociating cell agglomerations in the range of between >30 µm to 300 µm after harvest into single cells or cell oligomers in the range of 8-30 µm comprising the steps of* providing cell agglomerations in the range of between >30 µm to 300 µm* passing said cell agglomerations in the range of between >30 µm to 300 µm through a recirculation loop* bringing said cell agglomerations in the range of between >30 µm to 300 µm into contact with a shear component exerting a maximal shear in the range of between 2 Pa and 500 Pathereby dissociating the cell agglomerations in the range of between >30 µm to 300 µm into single cells or cell oligomers in the range of 8-30 µm.