INCUBATION METHOD AND SAMPLE HOLDER

A method is provided, comprising the steps of: providing a sample holder (300) having a flow channel (310) extending between a channel inlet (320) at an opening side (OS) and a channel outlet (320) at the opening side (OS) and defining a ceiling (312) associated with the opening side (OS); loading,...

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Bibliographische Detailangaben
Hauptverfasser: YU, Zhongjie, DAVOLI, Serena Alba, DE GROOT, Mattijs, RIJO DA COSTA CARVALHO, Rui Pedro, GREGG, Trillian Ashley, VAN LOENHOUT, Marinus Theodorus Johannes
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:A method is provided, comprising the steps of: providing a sample holder (300) having a flow channel (310) extending between a channel inlet (320) at an opening side (OS) and a channel outlet (320) at the opening side (OS) and defining a ceiling (312) associated with the opening side (OS); loading, in an upright orientation of the sample holder (300) in which the opening side (OS) is upward, the flow channel (310) with a liquid containing first cellular objects (330); forming a layer of the first cellular objects on the ceiling, comprising - inverting the sample holder into an upside-down orientation, and - incubating the first cellular objects for a defined period in the upside-down orientation to promote binding of least some of the cellular objects to the ceiling; and arranging, after the incubation, the sample holder in the upright orientation for a further use. Associated sample holders are also provided.