METHOD FOR TESTING EXPRESSION LEVEL OF PLURIPOTENCY GENE

The present invention relates to the use of a reference cell for testing pluripotency gene expression level, wherein the reference cell is selected from cells with low and stable expression level of a pluripotency gene. Preferably, the reference cell is selected from HFF cells, optionally, the pluri...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG, Yu, LUO, Yanchao, XU, Ranran, WANG, Shuyan, GAN, Yidi, ZHANG, Shuai
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to the use of a reference cell for testing pluripotency gene expression level, wherein the reference cell is selected from cells with low and stable expression level of a pluripotency gene. Preferably, the reference cell is selected from HFF cells, optionally, the pluripotency gene is selected from OCT4, NANOG. The present invention also relates to primers and probes for OCT4 and NANOG. The present invention also relates to a method for testing pluripotency gene expression level. The present invention further relates to a use of the detection method in detecting a hPSC residue level and characterizing a hPSC differentiation process. The method of the present invention has the advantages of simplicity, stability, high sensitivity, wide application range, and high accessibility. Therefore, it is easy to standardize and can provide a unified standard for testing cell pluripotency gene expression level.