COMPOSITIONS AND METHODS FOR END TO END CAPTURE OF MESSENGER RNAS

The subject matter disclosed herein is generally directed to methods and compositions for a single- or multi-pot protocol for the efficient end to end capture of RNAs (inclusive of their poly- A tail or their 3' end). The invention includes the use of capture oligonucleotides containing a 3...

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Bibliographische Detailangaben
Hauptverfasser:	ZWIRKO, Zachary, AL KHAFAJI, Aziz, HACOHEN, Nir
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The subject matter disclosed herein is generally directed to methods and compositions for a single- or multi-pot protocol for the efficient end to end capture of RNAs (inclusive of their poly- A tail or their 3' end). The invention includes the use of capture oligonucleotides containing a 3' non-extendable end and a selectively cleavable base upstream of an oligo-dT or oligo-dN and a 5' sequence containing unique molecular identifiers, and 2) a deoxyuracil glycosylase that acts only on a deoxyuracil present in a DNA:DNA duplex or DNA/RNA heteroduplex. The invention also includes the use of a dual template switching mechanism..