CELL CULTURE SYSTEM, METHOD FOR USING CELL CULTURE SYSTEM, AND CELL CULTURE UNIT

Provided is a cell culture system that can culture cells by providing a culture bag, is allowed to be easily transported with various tools necessary for the culture in one package and can utilize the culture bag for observation with a general-purpose microscope. This is a cell culture system for culturing cells using the culture bag made of soft packaging material, comprising a culture unit having a cutout portion on which a bag housing component having a pressing member that presses the culture bag is mounted, wherein a bag placed on the bag housing component, a medium vessel supplying a medium to the bag, a waste liquid vessel into which the medium is removed from the bag, a pumping means provided in a member connecting the bag and the medium vessel, and a pumping means provided in a member connecting the bag and the waste liquid vessel are provided on the unit, wherein a distance from a bottom surface of the unit to a culture surface is shorter than 20 mm, a liquid thickness of the medium becomes uniform when the pressing member presses the bag such that a surface of the unit, an upper surface and a lower surface of the pressing member as well as an upper surface and a culture surface of the bag become approximately parallel to one another.