PRODUCTION OF ALPHA-1,3-FUCOSYLATED COMPOUNDS

PRODUCTION OF ALPHA-1,3-FUCOSYLATED COMPOUNDS

This disclosure is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The disclosure describes methods for the production of a fucosylated compound using a fucosyltransferase as well as the purification of the fucosylated compound, the fucosyltransferase having...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: VERCAUTEREN, Annelies, VANDEWALLE, Kristof, BEAUPREZ, Joeri
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
SUGARS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:This disclosure is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The disclosure describes methods for the production of a fucosylated compound using a fucosyltransferase as well as the purification of the fucosylated compound, the fucosyltransferase having alpha-1,3-fucosyltransferase activity on the N-acetylglucosamine (GlcNAc) and/or the glucose (Glc) residue of Gal-β1,m-GlcNAc-β1,n-Gal-β1,4-Glc of a saccharide substrate comprising Gal-β1,m-GlcNAc-β1,n-Gal-β1,4-Glc wherein the m is 3 or 4 and the n is 3 or 6. The disclosure also provides a cell for production of a fucosylated compound. Next, the disclosure describes methods for the production of 3-fucosyllactose (3-FL) using a fucosyltransferase having alpha-1,3-fucosyltransferase activity on the Glc residue of lactose, as well as the purification of the 3-FL. The disclosure also provides a cell for production of 3-FL.