DISPLAY SUBSTRATE AND MANUFACTURING METHOD THEREOF, AND DISPLAY DEVICE

A display substrate (100) and a method of manufacturing the same, and a display device (200) are provided. The display substrate (100) includes: a base substrate (10) and a plurality of pixel units (PX) each includes a plurality of sub-pixels, and each sub-pixel includes a light emitting element and a pixel driving circuit; a functional layer (20) arranged on a side of the base substrate (10); a light-shielding layer (30) arranged on a side of the functional layer (20) away from the base substrate (10), wherein the light-shielding layer (30) is electrically connected to the functional layer (20); a buffer layer (34) arranged on a side of the light-shielding layer (30) away from the base substrate (10) and covering the functional layer (20) and the light-shielding layer (30); an active layer (40) arranged on a side of the buffer layer (34) away from the base substrate (10); a gate electrode layer (50) arranged on a side of the active layer (40) away from the base substrate (10); a first via hole (60) penetrating the buffer layer (34); and a signal line arranged on a side of the buffer layer (34) away from the base substrate (10). The functional layer (20) of the pixel driving circuit is electrically connected to the signal line through the first via hole (60). A projection of the light-shielding layer (30) on the base substrate (10) and a projection of the functional layer (20) on the base substrate (10) form an overlap region, and a projection of the first via hole (60) on the base substrate (10) is located in the overlap region.