MULTIPLEX ASSEMBLY OF NUCLEIC ACID MOLECULES

Compositions and methods are provided herein for high throughput synthesis and assembly of nucleic acid molecules. Specific aspects include methods and rules for designing, grouping and pooling of nucleic acid molecules for efficient multiplex assembly, amplification, processing and analysis to obta...

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Hauptverfasser:	KONOVALOVA, Tatiana, KUHN, Phillip, NETUSCHIL, Nikolai, HOFMEISTER, Thomas, PUETZSCHLER, Joern
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Compositions and methods are provided herein for high throughput synthesis and assembly of nucleic acid molecules. Specific aspects include methods and rules for designing, grouping and pooling of nucleic acid molecules for efficient multiplex assembly, amplification, processing and analysis to obtain error-free assembly products. Provided compositions and methods allow for miniaturization, parallelization, high throughput production and scaling, and cost reduction in gene synthesis workflows.