OFF-TARGET BLOCKING SEQUENCES TO IMPROVE TARGET DISCRIMINATION BY POLYMERASE CHAIN REACTION

Provided herein are methods and kits for discriminating a target sequence from a reference sequence in a polymerase chain reaction (PCR) by use of a promiscuity-blocking nucleotide juror (PBNJ) oligonucleotide.

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Bibliographische Detailangaben
Hauptverfasser:	KANE, Sarah, BARBARI, Stephanie, NASH, Rose, RAVET, Karl
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided herein are methods and kits for discriminating a target sequence from a reference sequence in a polymerase chain reaction (PCR) by use of a promiscuity-blocking nucleotide juror (PBNJ) oligonucleotide.