COMPOSITIONS AND METHODS FOR ISOLATING PROTEINS

COMPOSITIONS AND METHODS FOR ISOLATING PROTEINS

Systems and methods are described in which proteins are isolated from complex solution using successive chromatographic separations that retain the protein of interest in the flow-through. At least one of the chromatography media used is selected to be capable of interacting with both contaminants a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: RADTKE, Klaus Peter, ZURLO, Eugene, CURTIN, Dennis, DORFMAN, Ryan, WHELIHAN, Matthew
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
TRANSPORTING
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Beschreibung
Zusammenfassung:Systems and methods are described in which proteins are isolated from complex solution using successive chromatographic separations that retain the protein of interest in the flow-through. At least one of the chromatography media used is selected to be capable of interacting with both contaminants and the protein of interest, however capacity of this media is selected such that the protein of interest is displaced and remains in the flow-through.