TRANSAMINASE MUTANT AND USE THEREOF

Disclosed are a transaminase mutant and the use thereof, herein the transaminase mutant has a sequence formed after an amino acid mutation occurs in a sequence as shown in SEQ ID NO: 1, and the site at which the amino acid mutation occurs includes a V242W site. On the basis of transaminase shown in...

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Bibliographische Detailangaben
Hauptverfasser:	JAMES, Gage, LI, Rui, HONG, Hao, ZHANG, Yanqing, JIAO, Xuecheng, ZHANG, Na, YANG, Yiming
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed are a transaminase mutant and the use thereof, herein the transaminase mutant has a sequence formed after an amino acid mutation occurs in a sequence as shown in SEQ ID NO: 1, and the site at which the amino acid mutation occurs includes a V242W site. On the basis of transaminase shown in SEQ ID NO: 1, the transaminase mutant undergoes the mutation by means of a site-directed mutation method, thereby the amino acid sequence of the transaminase is changed and changes in protein structure and function are achieved, and then the transaminase with the above mutation site is obtained by means of a directed screening method. The transaminase obtained has relatively high catalytic activity, specific selectivity, wide substrate spectrum and broad industrial prospect.