METHOD FOR MASS ISOLATION AND CONCENTRATION OF TISSUE-DERIVED VESICLES

The present disclosure relates to a method of separating and concentrating a large number of extracellular vesicles (EVs) from various tissues, and more particularly, to a method of quickly separating and concentrating a large number of extracellular vesicles to be separated from animal and human ti...

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Bibliographische Detailangaben
Hauptverfasser: YOON, Song Hee, LEE, Sam Yeon
Format: Patent
Sprache:eng ; fre ; ger
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Beschreibung
Zusammenfassung:The present disclosure relates to a method of separating and concentrating a large number of extracellular vesicles (EVs) from various tissues, and more particularly, to a method of quickly separating and concentrating a large number of extracellular vesicles to be separated from animal and human tissues using multiple washing processes, enzyme reactions, and filters.According to the method of separating and concentrating a large number of tissue-derived extracellular vesicles (exosomes and ectosomes) of the present disclosure, compared to conventional exosome isolation kits or ultra-high-speed centrifugation methods, extracellular vesicles from tissues may be rapidly separated and concentrated in large quantities.