POLYMERASE CHAIN REACTION WELL INCLUDING A SIDE WALL WITH A FLUOROPOLYMER
A device includes at least one well to receive a polymerase chain reaction (PCR) mixture with the at least one well including a bottom and side walls. The bottom includes an electrically resistive sheet including at least one opening and to receive a signal from a signal source to generate heat to c...
Gespeichert in:
1. Verfasser: | |
---|---|
Format: | Patent |
Sprache: | eng ; fre ; ger |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | A device includes at least one well to receive a polymerase chain reaction (PCR) mixture with the at least one well including a bottom and side walls. The bottom includes an electrically resistive sheet including at least one opening and to receive a signal from a signal source to generate heat to cause a pulse-controlled amplification, thermal cycling zone in close thermal proximity to the resistive sheet. The side walls comprise a polymer material and a fluoropolymer component to cause the PCR mixture to form a contact angle of at least 45 degrees relative to the side walls. An optical detector is alignable with the at least one opening to detect fluorophores as an output of the PCR mixture. |
---|