FUSION PROTEIN FOR TREATING MYASTHENIA GRAVIS

The invention relates to a method of producing a fusion protein between an extracellular domain of neuronal acetylcholine receptor subunit alpha 1 (nAChR&1) and a solubility enhancing peptide. The method comprises solubilizing inclusion bodies comprising the fusion protein in a solubilization so...

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Bibliographische Detailangaben
Hauptverfasser:	FRIBERT, Charlotte, CHRISTISON, Richard Ian, ANDERSSSON, Jan-Olof, LÖWENADLER, Björn, SJÖHAMN, Jennie
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	CHEMISTRY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
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Beschreibung
Zusammenfassung:	The invention relates to a method of producing a fusion protein between an extracellular domain of neuronal acetylcholine receptor subunit alpha 1 (nAChR&1) and a solubility enhancing peptide. The method comprises solubilizing inclusion bodies comprising the fusion protein in a solubilization solution having a pH of at least 11 to form solubilized fusion proteins. The method also comprises diluting the solubilized fusion protein in a refolding solution having a pH of no more than 9 to form a refolded monomeric form of the fusion protein.