A GENETICALLY ENGINEERED BACTERIUM AND A PREPARATION METHOD AND USE THEREOF

A genetically engineered bacterium and a preparation method and use thereof are disclosed. The genetically engineered bacteria contain a gene encoding α-1,2-fucosyltransferase, and a gene encoding a protein tag is connected to the gene encoding α-1,2-fucosyltransferase; the protein tag is MBP, SUMO1...

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Bibliographische Detailangaben
Hauptverfasser:	WU, Yan, TANG, Jing, ZHAO, Jin, LIU, Yurou, XU, Hong
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A genetically engineered bacterium and a preparation method and use thereof are disclosed. The genetically engineered bacteria contain a gene encoding α-1,2-fucosyltransferase, and a gene encoding a protein tag is connected to the gene encoding α-1,2-fucosyltransferase; the protein tag is MBP, SUMO1, SUMO2 or TrxA, the amino acid sequence of the MBP is shown in SEQ ID NO: 2, the amino acid sequence of the SUMO1 is shown in SEQ ID NO: 3, the amino acid sequence of the SUMO2 is shown in SEQ ID NO: 4, the amino acid sequence of the TrxA is shown in SEQ ID NO: 5. Fermentation with the genetically engineered bacteria can greatly increase the yield of 2′-fucosyllactose compared to the genetically engineered bacteria that only expresses α-1,2-fucosyltransferase exogenously, and the yield can be more than doubled in a preferred case.