METHOD AND DEVICE FOR ANALYZING A BIOLOGICAL SAMPLE

A method for analyzing a biological sample (1002) comprises: Providing a plurality of markers (1612), each marker (1300 to 1309, 1400 to 1422, 1500 to 1508, 1518, 1526) comprising a fluorescent dye (1320) unique to the marker (1300 to 1309, 1400 to 1422, 1500 to 1508, 1518, 1526) and an affinity reagent (1310 to 1319) unique to the marker (1300 to 1309, 1400 to 1422, 1500 to 1508, 1518, 1526), the affinity reagent (1310 to 1319) being configured to attach to a predetermined structure (1706 to 1714) within the sample (1002). Staining the sample (1002) by introducing the plurality (1612) of markers into the sample (1002). Directing first excitation light having a first wavelength spectrum onto the sample (1002) in order to excite the fluorescent dyes (1320) of a first set of markers (1614). Generating at least one first image from fluorescence light emitted by the excited dyes of the first set (1614), the first image comprising at least two channels, each channel corresponding to one marker (1300 to 1309, 1400 to 1422, 1500 to 1508, 1518, 1526) of the first set of markers (1614). Directing at least one second excitation light having a second wavelength spectrum onto the sample (1002) in order to excite the fluorescent dyes (1320) of a second set of markers (1616), the second set (1616) being distinct from the first set (1614). Generating at least one second image from fluorescence light emitted by the excited dyes of the second set (1616), the second image comprising at least two channels, each channel corresponding to one marker (1300 to 1309, 1400 to 1422, 1500 to 1508, 1518, 1526) of the second set of markers (1616).