METHOD FOR OPERATING A BIOPROCESS INSTALLATION FOR PRODUCTION OF A BIOPRODUCT

The invention relates to a method for operating a bioprocess installation (1) for production of a bioproduct, wherein the bioprocess installation (1) comprises a source receptacle (2) for cell cultivation, a harvest receptacle (3) for bioproduction and a clarification setup (4) with a centrifuge (12), wherein the source receptacle (2) is operated in a cyclical production mode comprising the steps of:a) starting the cyclical production mode in the source receptacle (2)b) cultivating the cells in the source receptacle (2), thereby obtaining a cell broth (7) comprising cultivated cells,c) discharging a discharge fraction (16) of the cell broth (7) from the source receptacle (2),d) combining a restart fraction (17) of the cell broth (7) with fresh cultivation medium (8) and repeating step b),e) repeating steps c) and d) at least once and/orf) discharging the cell broth (7) obtained from step d) from the source receptacle (2) stopping the cyclical production mode, obtaining a discharge fraction (16),wherein the method further comprises the steps of:i) centrifuging the discharge fraction (16) via the centrifuge (12), thereby separating the discharge fraction (16) into at least a centrifuged discharge fraction (19) and supernatant (14) and preferably bioproduct,ii) operating the harvest receptacle (3) in a production mode,wherein steps i) and ii) are executed at least twice.