SYSTEMS AND METHODS FOR CELL-FREE POLYPEPTIDE SYNTHESIS

Disclosed herein is a method for cell-free polypeptide synthesis. The method comprises lysing a cell to produce a cellular lysate, passing the cellular lysate through a filter to produce a cellular extract, contacting the cellular extract with a nucleic acid encoding a polypeptide to produce a react...

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Hauptverfasser: YOESEP, Danielle, Jessica, SHAH-MORALES, Sajita, Caroni, KIGHTLINGER, Weston, Kevin
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:Disclosed herein is a method for cell-free polypeptide synthesis. The method comprises lysing a cell to produce a cellular lysate, passing the cellular lysate through a filter to produce a cellular extract, contacting the cellular extract with a nucleic acid encoding a polypeptide to produce a reaction mixture, and incubating the reaction mixture to produce the polypeptide.